LRRC1在乳腺癌组织中的表达及与Wnt/β-catenin信号通路关键转录因子TCF7L2的关系  

Expression of LRRC1 in breast cancer tissues and its relationship with TCF7L2,a key transcription factor of the Wnt/β-catenin signaling pathway

作　　者：张芳芳 韩一菲 王靖楠 邹炎 李娜[1] ZHANG Fangfang;HAN Yifei;WANG Jingnan;ZOU Yan;LI Na(Department of Pathology,Xinxiang Medical University,Henan Xinxiang 453003,China;Department of Pathology,Thrid Affiliated Hospital of Xinxiang Medical University,Henan Xinxiang 453003,China;Sanquan College of Xinxiang Medical University,Henan Xinxiang 453003,China)

机构地区：[1]新乡医学院病理学系,河南新乡453003 [2]新乡医学院第三附属医院病理科,河南新乡453003 [3]新乡医学院三全学院,河南新乡453003

出　　处：《现代肿瘤医学》2024年第20期3868-3874,共7页Journal of Modern Oncology

基　　金：河南省教育厅高校重点科研项目(编号:24A310010);新乡医学院基础医学院科研培育项目(编号:JCYXYKY202017)。

摘　　要：目的:探讨乳腺癌中富含亮氨酸的重复序列蛋白1(leucine-rich repeat-containing protein 1,LRRC1)和转录因子7类似物2(transcription factor 7-like 2,TCF7L2)的表达与临床病理特征的关系,并分析二者之间的相关性及其在乳腺癌病理诊断中的价值。方法:UALCAN数据库分析LRRC1 mRNA和蛋白在乳腺癌中的表达及与患者临床病理特征的关系;Kaplan-Meier Plotter数据库分析LRRC1的表达对乳腺癌患者预后的影响;The Human Protein Atlas(HPA)数据库分析LRRC1和TCF7L2在乳腺癌中的表达和定位;GEPIA数据库预测在乳腺癌中LRRC1与TCF7L2表达之间的相关性;免疫组织化学SP法检测乳腺癌和癌旁正常乳腺组织中LRRC1和TCF7L2蛋白的表达,分析LRRC1和TCF7L2蛋白与乳腺癌临床病理特征的关系,并采用Spearman分析二者之间的相关性;受试者工作特征曲线(receiver operating characteristic curve,ROC曲线)评估LRRC1和TCF7L2在乳腺癌病理诊断中的价值。结果:数据库分析结果表明,LRRC1 mRNA和蛋白在乳腺癌组织中的表达显著高于正常乳腺组织(P<0.05),其在乳腺癌组织中的表达水平与不同淋巴结转移分组和乳腺癌分子分型密切相关,并且LRRC1的表达与乳腺癌患者的总生存期(overall survival,OS)和无进展生存期(progression free survival,PFS)均有显著差异(P<0.05),LRRC1和TCF7L2表达水平之间存在显著相关性(P<0.05);免疫组化结果显示,LRRC1和TCF7L2蛋白在乳腺癌组织中的表达均显著高于癌旁正常乳腺组织(P<0.05),LRRC1蛋白表达与肿瘤大小、分期和淋巴结转移均密切相关(均P<0.05),TCF7L2蛋白表达与肿瘤分级、分期和淋巴结转移均相关(均P<0.05);Spearman相关性分析显示,LRRC1和TCF7L2表达呈正相关(r=0.366,P<0.01);ROC曲线显示,LRRC1和TCF7L2的ROC曲线下面积(area under curve,AUC)分别为0.826和0.786,二者蛋白表达对乳腺癌具有较高的诊断价值。结论:LRRC1和TCF7L2与乳腺癌发生发展及预后密切相关,检测LRRC1�Objective:To investigate the relationship between the expression of leucine-rich repeat-containing protein 1(LRRC1)and transcription factor 7-like 2(TCF7L2)and clinicopathological features in breast cancer,and analyze the correlation between the two and their value in breast cancer pathology diagnosis.Methods:UALCAN database to analyze the expression of LRRC1 mRNA and protein in breast cancer and the relationship with clinicopathological characteristics of patients.Kaplan-Meier Plotter database to analyze the effect of LRRC1 expression on the prognosis of breast cancer patients.The Human Protein Atlas(HPA)database analyzes the expression and localization of LRRC1 and TCF7L2 in breast cancer.GEPIA database predicts the correlation between LRRC1 and TCF7L2 expression in breast cancer.Immunohistochemical SP method was used to detect the expression of LRRC1 and TCF7L2 proteins in breast cancer and paracancerous normal breast tissues,to analyze the relationship between LRRC1 and TCF7L2 proteins and the clinicopathological features of breast cancer,and to analyze the correlation between the two using Spearman's analysis.Receiver operating characteristic curve(ROC curve)to assess the value of LRRC1 and TCF7L2 in the pathologic diagnosis of breast cancer.Results:Database analysis showed that the expression of LRRC1 mRNA and protein in breast cancer tissues was significantly higher than that in normal breast tissues(P<0.05),and its expression level in breast cancer tissues was closely correlated with different lymph node metastasis subgroups and breast cancer molecular typing,and the expression of LRRC1 was significantly different from both overall survival(OS)and progression free survival(PFS)of breast cancer patients(P<0.05),and there was a significant correlation between the expression levels of LRRC1 and TCF7L2(P<0.05).Immunohistochemical results showed that the expression of both LRRC1 and TCF7L2 proteins in breast cancer tissues was significantly higher than that in normal breast tissues adjacent to the cancer(P<0.0

关 键 词：LRRC1 TCF7L2 乳腺癌 临床病理特征 诊断价值 

分 类 号：R737.9[医药卫生—肿瘤]