阿莫西林对厚壁菌门微生物生长及代谢的影响  

The effect of amoxicillin on the growth and metabolism of Firmicutes

作　　者：陈文悦 李伟明 李波涛 廉梅花 李莉霞 王少锋 曾祥峰[2] 贾永锋[2] CHEN Wenyue;LI Weiming;LI Botao;LIAN Meihua;LI Lixia;WANG Shaofeng;ZENG Xiangfeng;JIA Yongfeng(School of Environment,Dalian University of Technology,Dalian 116024,Liaoning,China;Key Laboratory of Pollution Ecology and Environmental Engineering,Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China;Key Laboratory of Wastewater Treatment Technology of Liaoning Province,Shenyang Ligong University,Shenyang 110159,China;Shandong Provincial Geomineral Engineering Exploration Institute(801 Institute of Hydrogeology and Engineering Geology,Shandong Provincial Bureau of Geology&Mineral Resources),Jinan 250014,China)

机构地区：[1]大连理工大学环境学院,工业生态与环境工程教育部重点实验室,辽宁大连116024 [2]中国科学院污染生态与环境工程重点实验室,中国科学院沈阳应用生态研究所,沈阳110016 [3]沈阳理工大学环境与化学工程学院,沈阳110159 [4]山东省地矿工程勘察院(山东省地质矿产勘查开发局八〇一水文地质工程地质大队),济南250014

出　　处：《生态学杂志》2024年第9期2917-2922,共6页Chinese Journal of Ecology

基　　金：辽宁省应用基础专项项目(2023JH2/101300001,2023JH2/101600007);国家自然科学基金(52200041);辽宁振兴人才计划(XLYC200192);中国科学院青年创新促进会项目(2020200)资助。

摘　　要：抗生素污染对微生物生态造成严重影响。发酵是厌氧条件下的重要生物过程,但其对于抗生素污染的响应仍有待研究。本研究以典型β⁃内酰胺类抗生素阿莫西林为例,探究了不同浓度抗生素对两种厚壁菌门产芽孢微生物———丁酸梭菌和凝结芽孢杆菌发酵代谢的影响,主要关注两株菌发酵过程中生长曲线、碳源消耗、有机酸产量和pH的变化。结果表明:阿莫西林对丁酸梭菌和凝结芽孢杆菌的生长和碳源消耗均有抑制作用,对细菌形态的影响较小,50 mg·L^(-1)阿莫西林使丁酸梭菌停止生长,而凝结芽孢杆菌在培养24 h后可继续生长。在有机酸代谢方面,阿莫西林对凝结芽孢杆菌影响更为显著。两株菌的丁酸、甲酸、乳酸和乙酸生产均受到抑制,但在添加低浓度(1 mg·L^(-1))阿莫西林时,凝结芽孢杆菌的乳酸产量显著提高。Antibiotic contamination has a serious impact on microbial ecology.Fermentation is an important biological process under anaerobic conditions,but its response to antibiotic contamination remains unclear.In this study,we investigated the effects of different concentrations of antibiotics on the fermentation metabolism of Clostridium butyricum and Bacillus coagulans,two spore⁃producing microorganisms of the thick⁃walled phylum Clostridium perfringens,using the typicalβ⁃lactam antibiotic amoxicillin as an example.We measured the changes of the growth curves,carbon source consumption,organic acid production,and pH of both strains of bacteria during the fermen⁃tation process.The results showed that amoxicillin inhibited the growth and carbon source consumption of both C.butyricum and B.coagulans,with less effect on bacterial morphology.50 mg·L^(-1)amoxicillin caused the growth of C.butyricum to stop,whereas B.coagulans could continue to grow after 24 h of incubation.In terms of organic acid metabolism,the effect of amoxicillin on B.coagulans was more significant.Butyric,formic,lactic and acetic acid production of both strains was inhibited,but lactic acid production in B.coagulans was significantly increased un⁃der the addition of low concentration(1 mg·L^(-1))of amoxicillin.

关 键 词：丁酸梭菌 凝结芽孢杆菌 抗生素 发酵代谢 

分 类 号：X172[环境科学与工程—环境科学]