暗褐脉柄牛肝菌发酵液多糖的分离纯化与结构表征  

Isolation,purification and structure characterization of the polysaccharide from the fermentation broth of Phlebopus portentosus

作　　者：潘章超 王勇[1,2,3] 吴莉[1,2,3] 曾念开[1,2,3] PAN Zhangchao;WANG Yong;WU Li;ZENG Niankai(Key Laboratory of Tropical Translational Medicine of Ministry of Education,Haikou,Hainan 571199,China;Hainan Provincial Key Laboratory for Research and Development on Tropical Herbs,Haikou,Hainan 571199,China;School of Pharmacy,Hainan Medical University,Haikou,Hainan 571199,China)

机构地区：[1]热带转化医学教育部重点实验室,海南海口571199 [2]海南省热带药用植物研究开发重点实验室,海南海口571199 [3]海南医学院药学院,海南海口571199

出　　处：《食品与机械》2024年第7期12-16,62,共6页Food and Machinery

基　　金：海南省自然科学基金高层次人才项目(编号:820RC623)。

摘　　要：[目的]高效利用暗褐脉柄牛肝菌资源,开发其多糖功能性食品。[方法]取暗褐脉柄牛肝菌菌丝发酵培养,低温减压浓缩,乙醇沉淀,DEAE-52纤维素柱色谱分离纯化,透析袋透析除去小分子杂质制备多糖。总糖含量采用苯酚—硫酸显色法测定,液质联用法测定单糖组成,紫外分光光度法判断是否存在核酸和蛋白等杂质;通过红外光谱和核磁共振波谱,对多糖的结构进行分析。[结果]制备的多糖总糖含量为84%,主要单糖种类有:葡萄糖、甘露糖、半乳糖和阿拉伯糖,其物质的量比为2.07∶1.95∶1.00∶1.58。红外光谱和核磁共振波谱分析具有多糖吸收峰,含α型吡喃糖。[结论]从暗褐脉柄牛肝菌发酵液中制备的多糖主要由4种单糖组成,结构中含α型吡喃糖。[Objective]This study aimed to efficiently utilize the resources of Phlebopus portentosus and develop its polysaccharide functional food.[Methods]The mycelium of P.portentosus was cultured and fermented,and the combined fermentation broth was concentrated at low a temperature and reduced pressure.Then,precipitated by ethanol,separated,purified by DEAE-52 cellulose column chromatography,and the polysaccharide was finally refined by dialysis to remove the small molecular impurities.The total sugar content was determined by phenol-sulfuric acid colorimetry,the monosaccharide composition was determined by liquid chromatography-mass spectrometry(LC-MS),and the impurities such as nucleic acid and protein were detected by ultraviolet spectrophotometer.The structure of polysaccharides was analyzed preliminarily by infrared spectroscopy(IR)and nuclear magnetic resonance spectroscopy(NMR).[Results]The content of total sugar was 84%.The main monosaccharides of the polysaccharide were glucose,mannose,galactose,and arabinose,the molar ratio of which was 2.07∶1.95∶1.00∶1.58.Obvious polysaccharide absorption peaks were shown in the infrared spectrum and nuclear magnetic resonance spectra,through which anα-type pyranose can be inferred in the polysaccharide.[Conclusion]The polysaccharides prepared from the fermentation broth of P.portentosus were mainly composed of four monosaccharides,the structure of which containedα-pyranose.

关 键 词：暗褐脉柄牛肝菌 发酵液 多糖 分离 纯化 结构表征 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]