南瓜多糖调控Nrf2/γ-GCS通路对缺氧/复氧心肌细胞损伤的影响  

Impact of pumpkin polysaccharides on hypoxia/reoxygenation-induced myocardial cell injury by regulating Nrf2/γ-GCS pathway

作　　者：郑婉 杨珊珊 林云 魏俊萍 颜亚妮 ZHENG Wan;YANG Shanshan;LIN Yun;WEI Junping;YAN Yani(Department of Cardiovascular Medicine,the First Affiliated Hospital of Hainan Medical University,Haikou 570102,China)

机构地区：[1]海南医科大学第一附属医院心血管内科,海口570102

出　　处：《中国免疫学杂志》2024年第9期1871-1875,共5页Chinese Journal of Immunology

摘　　要：目的:探讨南瓜多糖(PP)调控Nrf2/γ-GCS通路对缺氧/复氧(H/R)诱导的心肌细胞(H9C2)损伤的影响。方法:建立HR细胞模型,确定PP给药浓度,将H9C2细胞分为Control组、H/R组、南瓜多糖组(PP组,2 mg/L PP)、Nrf2抑制剂组(ML385组,2.0μmol/L ML385)、PP+ML385组(2.0 mg/L PP+2.0μmol/L ML385),分别检测H9C2细胞凋亡、线粒体膜电位变化、氧化应激(LDH、MDA、SOD、ROS)、炎症因子(TNF-α、IL-6、IL-1β)水平及Nrf2、γ-GCS蛋白表达。结果:与Control组比较,H/R组细胞形态发生变化,细胞损伤及线粒体膜电位下降,H9C2细胞活力、SOD及Nrf2、γ-GCS蛋白表达水平显著降低,细胞凋亡率、LDH、MDA、ROS及TNF-α、IL-6、IL-1β水平显著升高(P<0.05);与H/R组比较,PP组细胞损伤减轻,线粒体膜电位恢复,H9C2细胞活力、SOD及Nrf2、γ-GCS蛋白表达水平显著升高,细胞凋亡率、LDH、MDA、ROS及TNF-α、IL-6、IL-1β水平显著降低(P<0.05),而ML385与PP作用效果相反,ML385可逆转PP的抗氧化、抗炎作用。结论:PP上调Nrf2/γ-GCS通路发挥抗氧化、抗炎作用,减轻H/R诱导的H9C2细胞损伤。Objective:To investigate the impact of pumpkin polysaccharide(PP)on hypoxia/reoxygenation(H/R)-induced myocardial cell(H9C2)injury by regulating Nrf2/γ-GCS pathway.Methods:HR cell models were established,and the concentration of pumpkin polysaccharide(PP)was determined.H9C2 cells were grouped into Control group,H/R group,pumpkin polysaccharide group(PP group,2 mg/L PP),Nrf2 inhibitor group(ML385 group,2.0μmol/L ML385),and PP+ML385 group(2.0 mg/L PP+2.0μmol/L ML385),the apoptosis,mitochondrial membrane potential changes,oxidative stress(LDH,MDA,SOD,ROS),the levels of inflammatory factors(TNF-α,IL-6,IL-1β)and the protein expressions of Nrf2 andγ-GCS in H9C2 cells were detected,respectively.Results:Compared with Control group,H/R group had changes in cell morphology,the cell injury and mitochondrial membrane potential decreased,the H9C2 cell viability,SOD and protein expression levels of Nrf2 andγ-GCS were significantly decreased,while the apoptosis rate,LDH,MDA,ROS,and levels of TNF-α,IL-6 and IL-1βwere significantly increased(P<0.05);compared with H/R group,the cell injury in PP group was reduced,the mitochondrial membrane potential was restored,the H9C2 cell viability,SOD and protein expression levels of Nrf2 andγ-GCS were significantly increased,and the apoptosis rate,LDH,MDA,ROS,and levels of TNF-α,IL-6 and IL-1βwere significantly decreased(P<0.05),the effect of ML385 was opposite to that of PP,and ML385 could reverse the antioxidant and anti-inflammatory effects of PP.Conclusion:PP up-regulates the Nrf2/γ-GCS pathway to exert antioxidant and anti-inflammatory effects,and reduces H/R-induced H9C2 cell injury.

关 键 词：南瓜多糖 缺氧/复氧 心肌细胞损伤 Nrf2/γ-GCS通路 

分 类 号：R542.2[医药卫生—心血管疾病]