基于脂质组学的整合素αvβ6对结肠癌细胞的促癌机制研究  

Effects of integrin αvβ6 on lipidomics metabolism in relation to tumor progression for colon cancer

作　　者：梁本甲 付仰彬 于金涛 王军 牛军[3] 周明良 LIANG Ben-jia;FU Yang-bin;YU Jin-tao;WANG Jun;NIU Jun;ZHOU Ming liang(Department of Gastrointestinal Surgery,Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,China;Department of General Surgery,Zhangdian Renmin Hospital,Zibo 255020,China;Department of Hepatobiliary Surgery,Qilu Hospital of Shandong University,Jinan 250012,China)

机构地区：[1]山东第一医科大学附属省立医院胃肠外科,山东济南250021 [2]淄博市张店区人民医院普外科,山东淄博255020 [3]山东大学齐鲁医院肝胆外科,山东济南250012

出　　处：《中国现代普通外科进展》2024年第9期678-683,共6页Chinese Journal of Current Advances in General Surgery

基　　金：山东省自然科学基金(ZR2020MH219,ZR2023MH088)。

摘　　要：目的:探讨整合素αvβ6对结肠癌细胞的脂质代谢的影响;确定用于诊断结肠癌的潜在代谢生物标志物。方法:利用基于液相色谱-质谱(LC-MS)的脂质组学分析研究αvβ6对结肠癌细胞系SW480代谢变化的影响。偏最小二乘判别分析模型在αvβ6过表达或低表达的SW480中显示出不同的脂质特征。结果:在αvβ6过表达后,SW480细胞中共有252种脂质呈现出显著的差异,其中138种脂质出现上调、114种脂质出现下调。同时发现,甲基磷胆碱(MePC)、磷脂酰胆碱(PC)、磷酰乙醇胺(PE)和甘油三酯(TG)对αvβ6过表达的癌细胞具有良好的诊断潜力。结论:整合素αvβ6可能通过调节脂质代谢失调的途径,使癌细胞更具侵袭性和转移性。这些结果可能为αvβ6对结肠癌细胞作用的分子机制提供潜在的线索。Objective:To investigate the effect of integrinαvβ6 on lipid metabolism in colon cancer cells;to identify potential metabolic biomarkers for the diagnosis of colon cancer.Methods:Liquid chromatography-mass spectrometry(LC-MS)-based lipidomic analysis was utilized to investigate the effects ofαvβ6 on the changes in SW480 metabolism in colon cancer cell lines.The partial least squares discriminant analysis model showed different lipid profiles in Integrinαvβ6 over-expressed or low-expressed SW480.Results:After Integrinαvβ6 overexpression,a total of 252 lipids showed significant differences in SW480 cells,of which 138 showed up-regulation and 114 showed down-regulation.Moreover,the Methyl phosphatidylcholine(MePC),phosphatidyl-choline(PC),phosphatidylethanolamine(PE)and triglycerides(TG)were also found to have good diagnostic potential inαvβ6 overexpressing cancer cells.Conclusion:Integrinαvβ6 may promote cancer cell invasivness and metastasis by regulating pathways of dysregulated lipid metabolism.These results may provide potential clues to the molecular mechanisms of Integrinαvβ6 on colon cancer cells.

关 键 词：结肠肿瘤 整合素 αvβ6 脂质组学 脂质代谢 

分 类 号：R735.35[医药卫生—肿瘤]