薰衣草精油的精制分离与抗炎活性研究  

Refinement and seperation of lavender essential oil and its anti-inflammatory activity

作　　者：王倩 刘菲 杨素珍 郭凤娇 哈米提江·阿力木江 吴华[2] 魏梦雅 WANG Qian;LIU Fei;YANG Suzhen;GUO Fengjiao;HAMITIJIANG·Alimujiang;WU Hua;WEI Mengya(Shandong Freda Biotech Co.,Ltd.,Jinan 250101;College of Chemistry and Materials Engineering,Beijing Technology and Business University,Beijing 100048)

机构地区：[1]山东福瑞达生物股份有限公司,济南250101 [2]北京工商大学化学与材料工程学院,北京100048

出　　处：《中国食品添加剂》2024年第10期108-117,共10页China Food Additives

基　　金：山东省企业技术创新项目(202250101132)。

摘　　要：为探究作用时间及成分组成对薰衣草精油(LEO)的活性影响,采用气相色谱-质谱联用法(GC-MS)测定LEO及其精制分离后的轻、重馏分的化学成分,利用LPS诱导的巨噬细胞RAW264.7炎症模型,探究LEO的抗炎活性与作用时间的关系及其轻、重馏分的抗炎活性差异。结果显示,乙酸芳樟酯、芳樟醇、乙酸薰衣草酯等为LEO的主要成分;LEO的最佳作用时间为12 h,该条件下具有较高的抗炎活性。LEO的轻、重馏分均在抗炎活性上存在一定的差异性,其中在NO、SOD、IL-6指标改善方面,轻重馏分差异不显著;在ROS、CAT、TNF-α指标改善方面,重馏分显著优于轻馏分(P<0.01);在MDA指标改善方面,轻馏分显著优于重馏分(P<0.01)。以上结果表明,LEO的抗炎活性与其作用时间及成分组成和含量有关。To investigate the effects of exposure time and composition on the activity of lavender essential oil(LEO),the chemical compositions of LEO and its purified light and heavy fractions(LLEO and HLEO)after refinement and separation were detected by gas chromatography-mass spectrometry(GC-MS).LPS-induced RAW264.7 cells inflammation model was used to investigate the relationship between the anti-inflammatory activity and exposure time of LEO and the difference of anti-inflammatory activity between light and heavy fractions.The results showed that linalyl acetate,linalool and lavender acetate were main components of LEO.LEO's optimal exposure time was 12 h.Under this condition,LEO showed high anti-inflammatory activity.LLEO and HLEO had some differences on the oxidative stress markers and inflammatory factors in LPS-induced RAW 264.7 cells.There were no significant differences between LLEO and HLEO on NO secretion,SOD enzyme activities and IL-6 secretion in LPS-induced RAW 264.7 cells.HLEO was significantly better than the LLEO in reducing ROS level,improving CAT enzyme activities and inhibiting TNF-αsecretion(P<0.01).LLEO was significantly better than the HLEO in reducing MDA content(P<0.01).The above results showed that the anti-inflammatory activity of LEO was related to its action time,compositions and content.

关 键 词：薰衣草精油 分子蒸馏 气相色谱-质谱联用 氧化应激 抗炎活性 

分 类 号：TS225.3[轻工技术与工程—粮食、油脂及植物蛋白工程]