癌组织中MACC1、KIF23表达对早期结肠癌术后再发的预测价值  

Predictive Value of Expression of MACC1 and KIF23 in Cancer Tissues for Postoperative Recurrence of Early Colon Cancer

作　　者：常伟 CHANG Wei(Xinxiang First People's Hospital,Xinxiang,453000)

机构地区：[1]河南省新乡市第一人民医院,453000

出　　处：《实用癌症杂志》2024年第10期1630-1634,共5页The Practical Journal of Cancer

摘　　要：目的观察早期结肠癌患者癌组织内结肠癌转移相关基因-1(MACC1)、驱动蛋白家族成员23(KIF23)表达,并探讨二者对早期结肠癌术后再发的预测价值。方法选择2018年6月至2021年6月拟于新乡市第一人民医院接受腹腔镜联合纤维结肠镜手术的80例早期结肠癌患者,采用实时荧光定量RT-PCR法测定患者癌组织与癌旁组织中MACC1、KIF23 mRNA表达,经Pearson相关性分析MACC1、KIF23 mRNA关系。术后随访2年,根据是否再发分为再发组、未再发组,对比2组术前基础资料与癌组织MACC1、KIF23 mRNA表达,建立Logistic回归模型筛选早期结肠癌术后再发的影响因素,绘制ROC曲线分析术前癌组织MACC1、KIF23 mRNA表达预测早期结肠癌术后再发的价值。结果术前癌组织MACC1 mRNA、KIF23 mRNA相对表达量均高于癌旁组织(P<0.05);Pearson相关性发现,术前癌组织MACC1 mRNA与KIF23 mRNA相对表达量呈正相关(γ=0.326,P<0.05)。再发组肿瘤直径≥3 cm、合并梗阻、TNM分期为Ⅱ期的患者占比及MACC1 mRNA、KIF23 mRNA水平均高于未再发组(P<0.05);经Logistic回归分析,结果显示,肿瘤直径≥3 cm(OR=3.947,95%CI=1.256~12.409)、梗阻(OR=4.385,95%CI=1.273~15.101)、TNM分期Ⅱ期(OR=4.071,95%CI=1.058~15.666)、MACC1 mRNA高表达(OR=2.485,95%CI=1.126~7.325)、KIF23 mRNA高表达(OR=3.467,95%CI=1.298~9.415)是影响早期结肠癌术后再发的独立危险因素(OR>1,P<0.05);绘制ROC曲线发现,术前癌组织MACC1 mRNA、KIF23 mRNA及二者联合预测早期结肠癌术后再发的AUC为0.771(95%CI:0.649~0.894)、0.849(95%CI:0.722~0.976)、0.928(95%CI:0.825~1.000)。结论早期结肠癌患者癌组织内MACC1、KIF23呈高表达,且二者联合可有效预测早期结肠癌术后再发,临床应密切监测患者术前癌组织内MACC1、KIF23的表达水平,以筛查术后再发的高危人群。Objective To observe the expression of colon cancer metastasis-related gene-1(MACC1)and kinesin family member 23(KIF23)in cancer tissues of patients with early colon cancer,and investigate their predictive value for postoperative recurrence of early colon cancer.Methods 80 patients with early colon cancer who were scheduled to receive laparoscopic combined fibercolonoscopy were selected,and the mRNA expressions of MACC1 and KIF23 in the cancer tissues and adjacent tissues of the patients were determined by real-time fluorescence quantitative RT-PCR.The mRNA relationship between MACC1 and KIF23 was analyzed by Pearson correlation.After 2 years of postoperative follow-up,patients were divided into recurrence group and non-recurrence group according to whether recurrence occurred.The preoperative basic data of the 2 groups and the mRNA expression of MACC1 and KIF23 in cancer tissues were compared,and a Logistic regression model was established to screen the influencing factors of postoperative recurrence of early colon cancer.ROC curve was drawn to analyze the value of MACC1 and KIF23 mRNA expression in predicting early colon cancer and postoperative recurrence.Results The relative expression levels of MACC1 mRNA and KIF23 mRNA in preoperative cancer tissues were higher than those in paracancer tissues(P<0.05).Pearson correlation showed that the relative expression of MACC1 mRNA and KIF23 mRNA in preoperative cancer tissues was positively correlated(γ=0.326,P<0.05).The proportion of tumor diameter≥3 cm,obstruction,TNM stageⅡand MACC1 mRNA and KIF23 mRNA in recurrent group were higher than those in non-recurrent group(P<0.05).Logistic regression analysis showed that tumor diameter≥3 cm(OR=3.947,95%CI=1.256~12.409),obstruction(OR=4.385,95%CI=1.273~15.101),TNM stageⅡ(OR=4.071,95%CI=1.058~15.666),MACC1 mRNA high expression(OR=2.485,95%CI=1.126~7.325),KIF23 mRNA high expression(OR=3.467,95%CI=1.298~9.415)was an independent risk factor for postoperative recurrence of early colon cancer(OR>1,P<0.05).ROC curve foun

关 键 词：早期结肠癌 癌组织 结肠癌转移相关基因-1 驱动蛋白家族成员23 复发 

分 类 号：R735.3[医药卫生—肿瘤]