CENPA、GRHL2与乳腺癌临床病理特征的关系及其在预后评估中的临床价值  

Relationship between CENPA and GRHL2 and clinicopathological characteristics of breast cancer and its clinical value in prognosis evaluation

作　　者：王晶晶[1] 沈苏琴 WANG Jingjing;SHEN Suqin(Department of Clinical Laboratory,Women's Hospital Affiliated to Nanjing Medical University/Nanjing Maternal and Child Health Hospital,Nanjing,Jiangsu 210004,China)

机构地区：[1]南京医科大学附属妇产医院/南京市妇幼保健院检验科,江苏南京210004

出　　处：《国际检验医学杂志》2024年第19期2316-2322,共7页International Journal of Laboratory Medicine

摘　　要：目的探讨着丝粒蛋白A(CENPA)和粒状头样2(GRHL2)在乳腺癌组织中的表达,构建乳腺癌预后模型并分析其临床应用价值。方法采用基因表达谱交互分析(GEPIA)数据库分析1085例乳腺癌组织和291例正常组织中CENPA和GRHL2表达,Kaplan-Meier Plotter在线网站分析CENPA和GRHL2表达与乳腺癌患者生存情况的关系。选取2018年1月至2020年6月在该院接受治疗的84例原发性单侧乳腺癌患者作为研究对象,免疫组化法检测CENPA和GRHL2表达并分析其与乳腺癌临床病理特征的关系,Spearman相关分析CENPA、GRHL2与糖类抗原153(CA153)的相关性,受试者工作特征(ROC)曲线分析CENPA、GRHL2和CA153对乳腺癌预后评估的效能;Cox比例风险回归模型分析乳腺癌预后的危险因素。结果GEPIA数据库提示乳腺癌组织中CENPA、GRHL2表达水平高于正常组织(P<0.05);Kaplan-Meier Plotter分析表明,CENPA、GRHL2表达水平升高,患者生存率降低(Log-rank P<0.001);CENPA、GRHL2表达与CA153呈正相关(r=0.410、0.602,P<0.001),三者联合检测对乳腺癌诊断的曲线下面积为0.878;CENPA和GRHL2表达与乳腺癌患者TNM分期、组织学分级、淋巴结转移及表皮生长因子受体-2阳性有关(P<0.05);Cox回归分析显示,TNM分期Ⅲ期、组织学分级Ⅲ级、淋巴结转移及CENPA、GRHL2高表达是乳腺癌预后的独立危险因素(P<0.05)。结论CENPA和GRHL2是乳腺癌预后评估有价值的生物标志物,与CA153联合检测对乳腺癌预后具有较高的预测价值。Objective To investigate the expression of centromere protein A(CENPA)and granular head like 2(GRHL2)in breast cancer tissues,construct a prognostic model of breast cancer and analyze its clinical application value.Methods The expression of CENPA and GRHL2 in 1085 breast cancer tissues and 291 control tissues were analyzed using gene expression profiling interactive analysis(GEPIA)database.The relationship between CENPA and GRHL2 expression and the survival of breast cancer patients was analyzed using Kaplan-Meier Plotter online website.A total of 84 patients with primary unilateral breast cancer treated in the hospital from January 2018 to June 2020 were selected as the research objects.The expression of CENPA and GRHL2 was detected by immunohistochemistry,and the relationship between CENPA and GRHL2 and clinicopathological characteristics of breast cancer was analyzed.Spearman correlation analysis was used to analyze the correlation between CENPA,GRHL2 and carbohydrate antigen 153(CA153).Receiver operating characteristic(ROC)curve was used to analyze the prognostic value of CENPA,GRHL2 and CA153 in breast cancer.Cox proportional hazards regression model was used to analyze the risk factors of breast cancer prognosis.Results GEPIA database showed that the expression levels of CENPA and GRHL2 in breast cancer tissues were higher than those in normal tissues(P<0.05).Kaplan-Meier Plotter analysis showed that the expression levels of CENPA and GRHL2 increased,and the survival rate of patients decreased(Log-rank P<0.001).The expressions of CENPA and GRHL2 were positively correlated with CA153(r=0.410,0.602,P<0.001).The area under the curve of CENPA,GRHL2 and CA153 in breast cancer diagnosis was 0.878.The expressions of CENPA and GRHL2 were correlated with TNM stage,histological grade,lymph node me-tastasis and epidermal growth factor receptor-2 expression(P<0.05).Cox regression analysis showed that TNM stageⅢ,histological gradeⅢ,lymph node metastasis and high expression of CENPA and GRHL2 were independent prognos

关 键 词：乳腺癌 着丝粒蛋白A 粒状头样2 预测模型 

分 类 号：R737.9[医药卫生—肿瘤]