粪便miR-92a联合血清CEA、CA199、VEGF检测在结肠癌诊断中的价值分析  

Analysis of the value of fecal miR-92a combined with serum CEA,CA199 and VEGF detection in the diagnosis of colon cancer

作　　者：陈健康 苗杰 CHEN Jiankang;MIAO Jie(Deparment of Clinical Laboratory,the First Affiliated Hospital of Air Force Military Medical University,Xi'an,Shaanxi 710032,China;Deparment of Clinical Laboratory,Affiliated to Medical Support Center,970 Hospital of the People's Liberation Army Joint Logistic Support Force,Weihai,Shandong 264200,China)

机构地区：[1]空军军医大学第一附属医院检验科,陕西西安710032 [2]联勤保障部队第九七〇医院威海医疗区检验科,山东威海264299

出　　处：《国际检验医学杂志》2024年第19期2392-2395,共4页International Journal of Laboratory Medicine

摘　　要：目的探讨粪便微小RNA-92a(miR-92a)与血清癌胚抗原(CEA)、糖类抗原199(CA199)和血管内皮生长因子(VEGF)对结肠癌患者的诊断价值。方法选取2020年1月至2022年8月空军军医大学第一附属医院收治的结肠癌患者77例为结肠癌组,选取其中19例经腹腔镜根治术治疗的结肠癌患者于术后6个月门诊随访,另选取70例体检健康者为对照组及81例其他消化系统肿瘤患者为其他消化系统肿瘤组,采用实时荧光定量PCR检测患者粪便miR-92a水平,电化学发光法检测血清CEA、CA199水平,化学发光法检测血清VEGF水平,受试者工作特征(ROC)曲线分析miR-92a、CEA、CA199、VEGF对结肠癌的诊断价值。结果结肠癌组粪便miR-92a阳性率与其他消化系统肿瘤组及对照组比较,差异有统计学意义(P<0.001)。结肠癌组血清CEA、CA199和VEGF水平明显高于对照组(P<0.05)。相较术前,结肠癌患者miR-92a阳性率及血清CEA、CA199、VEGF水平在术后6个月均明显降低(P<0.05)。ROC曲线分析显示,CEA、CA199、VEGF、miR-92a联合检测的曲线下面积(AUC)最大,为0.901,灵敏度为77.9%,特异度为91.3%。miR-92a+CEA联合检测时,AUC为0.899,灵敏度为77.9%,特异度92.8%。结论miR-92a、CEA、CA199、VEGF检测均对结肠癌的诊断具有重要价值,miR-92a与CEA联合检测能够提高结肠癌诊断的灵敏度和特异度,并且对患者预后具有一定价值。Objective To investigate the diagnostic value of fecal microRNA-92a(miR-92a)and serum carcinoembryonic antigen(CEA),carbohydrate antigen 199(CA199)and vascular endothelial growth factor(VEGF)in patients with colon cancer.Methods A total of 77 patients with colon cancer admitted to the First Affiliated Hospital of Air Force Medical University from January 2020 to August 2022 were selected as the colon cancer group,including 19 patients who underwent laparoscopic radical resection of colon cancer and were followed up for 6 months after surgery.Another 70 healthy subjects were selected as the control group and 81 patients with other digestive system tumors were selected as the other digestive system tumors group.Realtime fluorescence quantitative PCR was used to detect the level of fecal miR-92a,electrochemiluminescence was used to detect the levels of serum CEA and CA199,and chemiluminescence was used to detect the level of serum VEGF.The receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of miR-92a,CEA,CA199 and VEGF in colon cancer.Results The positive rate of fecal miR-92a in colon cancer group was significantly higher than that in other digestive system tumors group and control group(P<0.001).The serum levels of CEA,CA199 and VEGF in colon cancer group were significantly higher than those in control group(P<0.05).The positive rate of miR-92a and the levels of serum CEA,CA199 and VEGF in colon cancer patients were significantly lower at 6 months after surgery than those before surgery(P<0.05).ROC curve analysis showed that the combined detection of CEA,CA199,VEGF and miR-92a had the largest area under the curve(AUC,0.901),sensitivity(77.9%)and specificity(91.3%).When miR-92a+CEA was combined,the AUC was 0.899,the sensitivity was 77.9%,and the specificity was 92.8%.Conclusion The detection of miR-92a,CEA,CA199 and VEGF has important value in the diagnosis of colon cancer.The combined detection of miR-92a and CEA can improve the sensitivity and specificity of the diagnosis of colo

关 键 词：微小RNA-92a 癌胚抗原 糖类抗原199 血管内皮生长因子 结肠癌 

分 类 号：R735.3[医药卫生—肿瘤]