PRDX2调控HPV16 E6对宫颈癌Siha细胞活性的影响  

Effect of PRDX2 regulation of HPV16 E6 on activity of cervical cancer Siha cells

作　　者：张林艳 郑荣荣[1] 姜文静 ZHANG Lin-yan;ZHENG Rong-rong;JIANG Wen-jing(Department of Gynecology,Jianhu Clinical Medical College,Yangzhou University,Yancheng 224700,China;Nantong University,Nantong 226000,China)

机构地区：[1]扬州大学建湖临床医学院妇科,江苏盐城224700 [2]南通大学,江苏南通226000

出　　处：《诊断病理学杂志》2024年第9期847-850,共4页Chinese Journal of Diagnostic Pathology

基　　金：建湖县卫健委指导性项目(编号:JY2902016);江苏医药职业学院校地协同创新研究项目(编号20239210)。

摘　　要：目的探究PRDX2在宫颈癌组织中的表达以及对宫颈癌Siha细胞的恶性生物学行为的影响。方法免疫组化方法检测宫颈癌组织以及癌旁组织中PRDX2的表达;将宫颈癌Siha细胞设置为4个组:Siha组、siRNA NC组、siRNA PRDX2组与siRNA HPV16 E6组。EDU染色法检测Siha细胞的生长活性;Transwell实验方法分析Siha细胞的侵袭数量;流式细胞术检测Siha细胞的凋亡情况;蛋白免疫印迹方法分析Siha细胞中PRDX2/HPV16 E6蛋白的表达水平。结果与宫颈癌旁组织比较,宫颈癌组织PRDX2的表达上调。与Siha组和siRNA NC组比较,siRNA PRDX2和siRNA HPV16 E6组的Siha细胞增殖能力下降(P<0.05);siRNA PRDX2和siRNA HPV16 E6组的Siha细胞侵袭数量下降(P<0.05);siRNA PRDX2和siRNA HPV16 E6组的Siha细胞凋亡率增加(P<0.05);siRNA PRDX2组的Siha细胞的HPV16 E6蛋白表达下调(P<0.05)。结论PRDX2在宫颈癌组织中表达增加,抑制PRDX2表达后,Siha细胞的增殖速度与侵袭数量降低,凋亡率增加,该机制与PRDX2调节HPV16 E6蛋白的表达相关。Objective To investigate the expression of PRDX2 in cervical cancer tissues and its effect on the malignant biological behavior of cervical cancer Siha cells.Methods The expression of PRDX2 in cervical cancer tissues and adjacent tissues was detected by immunohistochemistry.The cervical cancer Siha cells were divided into four groups:Siha group,siRNA NC group,siRNA PRDX2 group and siRNA HPV16 E6 group.The proliferation of Siha cells was detected by EDU staining assay.Transwell assay was used to analyze the invasion number of Siha cells.The apoptosis of Siha cells was detected by flow cytometry.The expression level of PRDX2/HPV16 E6 protein in Siha cells was analyzed by Western blot.Results Compared with the adjacent tissues,the expression of PRDX2 was up-regulated in cervical cancer tissues.Compared with Siha group and siRNA NC group,the proliferation ability of Siha cells in siRNA PRDX2 and siRNA HPV16 E6 group was decreased(P<0.05).The number of invasive Siha cells in siRNA PRDX2 and siRNA HPV16 E6 groups decreased(P<0.05).The apoptosis rate of Siha cells in siRNA PRDX2 and siRNA HPV16 E6 groups was increased(P<0.05).The expression of HPV16 E6 protein in Siha cells in the siRNA PRDX2 group was down-regulated(P<0.05).Conclusion The expression of PRDX2 is increased in cervical cancer tissues.After PRDX2 expression is inhibited,the proliferation rate and invasion number of Siha cells are decreased,and the apoptosis rate is increased.The mechanism is related to PRDX2 regulating the expression of HPV16 E6 protein.

关 键 词：PRDX2 增殖 HPV16 E6 侵袭 宫颈癌 转移 凋亡 SIHA 

分 类 号：R737.3[医药卫生—肿瘤]