PCK1和PCK2在结直肠癌中的表达及意义  

Expression of PCK1 and PCK2 in colorectal cancer and their significance

作　　者：付振鹏 王若曦 张帅帅 闫振礼 寇永亮 董玮[1] FU Zheng-peng;WANG Ruo-xi;ZHANG Shuai-shuai;YAN Zhen-li;KOU Yong-liang;TONG Wei(Department of Pathology,Weifang Hospital of Traditional Chinese Medicine,Weifang 261000,China;Department of Pathology,the People's Hospital of Weifang,Weifang 261000,China)

机构地区：[1]潍坊市中医院病理科,山东潍坊261000 [2]潍坊市人民医院病病理科,山东潍坊261000

出　　处：《诊断病理学杂志》2024年第9期866-871,共6页Chinese Journal of Diagnostic Pathology

基　　金：潍坊市卫生健康委员会科研项目(WFWSJK-2023-339)。

摘　　要：目的检测磷酸烯醇式丙酮酸羧激酶(PCK)在结直肠癌(CRC)组织中的表达,并分析其与CRC患者临床病理特征和预后的相关性。方法收集2016年至2019年在潍坊市中医院接受手术治疗的I-III期原发性CRC患者的癌组织和癌旁对照组织。采用免疫组化方法检测两种组织样本中PCK1和PCK2的表达,并分析CRC患者中PCK1和PCK2表达与患者临床病理特征和预后的相关性。结果CRC组织中PCK1和PCK2的表达均显著低于癌旁对照组织,其中有84.09%(259/308)的肿瘤检测出PCK1低表达,而76.30%(235/308)的肿瘤呈现PCK2低表达。PCK1低表达与肿瘤浸润(T分期)(P=0.001)和神经血管浸润(P=0.012)有关。PCK2低表达与患者年龄(P=0.035)、早期肿瘤分期(P=0.001)、淋巴结转移(P=0.001)和肿瘤浸润(T分期)(P=0.005)有关。预后分析显示,PCK1和PCK2表达与CRC预后没有相关性。结论PCK1和PCK2在CRC组织中低表达,可能与CRC的发生和进展密切相关。PCK1和PCK2可作为CRC治疗的潜在靶点。Objective To investigate the frequency and prognostic role of the phosphoenolpyruvate carboxykinase(PCK)in Chinese patients with colorectal cancer(CRC).Methods Clinicopathological and survival information from 308 patients with primary CRC were reviewed and recorded.PCK1 and PCK2 expression in CRC and adjacent normal tissues was analyzed by immunohistochemistry(IHC).The relationship between PCK1 and PCK2 expression and clinicopathological characteristics and outcomes were determined.Results The expression level of PCK1 and PCK2 in CRC tissues was significantly lower than that in adjacent normal tissues.Low PCK1 and PCK2 expression were detected in 84.09%(259/308)and 76.30%(235/308)patients with CRC,respectively.The expression of PCK1 was significantly correlated with tumor invasion(T stage)(P=0.001)and neurovascular infiltration(P=0.012).PCK2 expression was significantly correlated with age(P=0.035),TNM stage(P=0.001),lymph node metastasis(P=0.001)and tumor invasion(T stage)(P=0.005).PCK1 and PCK2 expression was not prognostic for disease-free survival and overall survival.Conclusion PCK1 and PCK2 was lowly expressied in CRC tissues and may be participated in the progress of CRC.Therefore,PCK1 and PCK2 might be potential targets for CRC treatment.

关 键 词：结直肠癌 磷酸烯醇式丙酮酸羧激酶 临床病理特征 免疫组织化学 

分 类 号：R730.56[医药卫生—肿瘤]