ABO血型抗体缺失致ABO血型正反定型不符的检测分析  

Detection and analysis of ABO blood group forward and reverse typing inconsistency caused by ABO blood group antibody deficiency

作　　者：秦洪伟 许梦迪 薛杰 QIN Hongwei;XU Mengdi;XUE Jie(Department of Blood Transfusion,Jiaozhou Central Hospital of Qingdao,Qingdao,266300,China)

机构地区：[1]青岛市胶州中心医院输血科,山东青岛266300

出　　处：《临床血液学杂志》2024年第8期545-549,共5页Journal of Clinical Hematology

摘　　要：目的:分析ABO血型反定型抗体缺失样本的血型血清学及基因检测结果,探讨ABO血型抗体缺失的原因和输血策略。方法:采用Ortho AutoVue全自动血型系统对该院2019年11月至2023年10月33296例患者进行常规ABO血型检测,选择ABO血型正反定型不符疑为抗体缺失者,采用盐水试管法复核并增加ABO亚型检测、吸收放散试验检测红细胞上的A或B抗原,对ABO基因第6和第7外显子进行测序分析。结果:95例ABO血型正反定型不符样本中,7例疑为血型抗体缺失,其中4例确认为ABO血型抗原减弱,3例确认为ABO血型抗体缺失,本地区ABO血型抗体缺失检出率0.09‰,经过基因测序分析,确定血型抗体缺失者基因型分别为B.01/O.01.02、O.01.13/O.01.13、B.01/O.01.01。结论:疑似ABO血型抗体缺失者需要从正定型和反定型分别进行检测分析,ABO基因检测可明确具体ABO基因型,提高ABO血型鉴定的准确性并可明确ABO血型抗体缺失现象。对于疑似或确定为ABO血型抗体缺失者的输血,可采用相容性输血的方式。Objective: To analyze the serological and genetic test results of samples with ABO reverse typing antibody deficiency,and to explore the causes of ABO reverse typing antibody deficiency and transfusion strategy.Methods: The Ortho AutoVue automatic blood group system was used to perform routine ABO blood group testing for 33296 patients in the hospital from November 2019 to October 2023.Those who were suspected of antibody deficiency due to the inconsistency of ABO blood group forward and reverse typing were selected.ABO subgroup detection,A or B antigen detection on red blood cells,and exon 6 and 7 of ABO gene sequencing were performed.Results: Among the 95 cases of ABO blood group positive and reverse typing discrepancies,7 cases were suspected to be ABO blood group antibody deficiency,of which 4 cases were confirmed to be ABO blood group antigen weakening and 3 cases were confirmed to be ABO blood group antibody deficiency.The detection rate of ABO blood group antibody deficiency in this region was 0.09‰.After gene sequencing analysis,the genotypes were B.01/O.01.02、O.01.13/O.01.13、B.01/O.01.01.Conclusion: Suspected ABO blood group antibody deficiency needs to be detected and analyzed from forward typing and reverse typing respectively.ABO gene testing can clarify the specific ABO genotype,improve the accuracy of ABO blood group identification and identify the phenomenon of ABO blood group antibody deficiency.Compatible transfusion can be used for patients who were suspected or confirmed ABO blood group antibody deficiency.

关 键 词：ABO血型 ABO反定型 血型抗体缺失 血型血清学 基因测序 

分 类 号：R457.1[医药卫生—治疗学]