可注射釉基质蛋白水凝胶制备及其性能研究  

Preparation and characterization of an injectable enamel matrix protein hydrogel

作　　者：王春宝 曾子瑜 全雅琦 王忠山 WANG Chunbao;ZENG Ziyu;QUAN Yaqi;WANG Zhongshan(College of Chemistry and Bio-engineering,Yichun University,China,336000;12th Team,Third Brigade,College of Basic Medicine,Air Force Medical University,Xi′an;State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Key Laboratory of Stomatology,Department of Prosthodontics,The Third Hospital Affiliated of Air Force Military Medical University,Xi'an)

机构地区：[1]宜春学院化学与生物工程学院,336000 [2]空军军医大学基础医学院学员三大队十二队 [3]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,第四军医大学口腔医院口腔修复科

出　　处：《实用口腔医学杂志》2024年第5期614-618,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金青年项目(编号:81700930);福建省自然科学基金青年创新项目(编号:2019J05117);江西省教育厅科研项目(编号:GJJ190840);陕西省重点研发计划一般项目(编号:2021SF-105)。

摘　　要：目的:构建一种负载釉基质蛋白(EMP)的泊洛沙姆(Poloxamer)可注射温敏水凝胶,研究其性能。方法:提取6月龄幼猪牙胚EMP,制备空白凝胶以及负载釉基质蛋白(2 mg/mL)的凝胶(P407∶P188分别为23∶8和19∶1),采用试管倒置法测定胶凝温度,超微量蛋白定量法测定优选组方的EMP释放性能。结果:负载2 mg/mL EMP,P407∶P188为23∶8的泊洛沙姆可注射温敏水凝胶(编号FP1)胶凝温度为33℃,其释放性能符合Higuchi方程,适合临床应用。结论:构建了可缓释EMP的泊洛沙姆可注射温敏水凝胶,该体系具有合适的相转变温度和控释效能。Objective:To prepare an enamel matrix protein(EMP)loaded poloxamer thermosensitive injectable hydrogel,and to study its characteristics.Methods:EMP was extracted from a 6-month old porcine and identified by SDS-PAGE electrophoresis.EMP at 2 mg/mL was loaded into gels poloxamer with P407∶P188 of 23∶8 and 19∶1 respectively.The gelling temperature of blank gel and EMP gel was detected by an inverted tube method.The release performance of selected EMP-loaded gel was detected by an Ultra-micro spectrophotometer.Results:A hydrogel(code number:FP1)with formula of 2 mg/mL EMP and 23∶8 of P407∶P188 possessed gelling temperature of 33℃,and the release behavior of FP1 conformed to the Higuchi equation.Conclusion:A poloxamer thermosensitive injectable hydrogel was successfully fabricated,this formulation possesses adaptive phase inversion temperature and controlled release efficiency.

关 键 词：泊洛沙姆 温敏可注射水凝胶 釉基质蛋白 释放性能 

分 类 号：R781.4[医药卫生—口腔医学]