MCP-1/CCR2轴介导NF-κB信号途径对致炎因素刺激的牙髓干细胞迁移的影响  

The influence of MCP-1/CCR2 axis mediated NF-κB signal pathway on the migration of dental pulp stem cells steamulated by inflamation factor

作　　者：李伟 彭建安 杨赣军 胡红梅 LI Wei;PENG Jian'an;YANG Ganjun;HU Hongmei(Oral dept of Medical College,Jinggangshan University,China,343000;Department of histology and anatomy of medical college,Jinggangshan University,Ji'an)

机构地区：[1]井冈山大学医学部口腔系,343000 [2]井冈山大学医学部组织学与解剖学教研室

出　　处：《实用口腔医学杂志》2024年第5期638-646,共9页Journal of Practical Stomatology

基　　金：江西省自然科学基金(编号:20202BAB206037);江西省卫健委科技计划项目(编号:202130707)。

摘　　要：目的:研究MCP-1/CCR2轴在介导NF-κB信号途径影响致炎因素刺激的牙髓干细胞(DPSCs)迁移的分子机制。方法:取SD大鼠进行DPSCs原代培养,流式细胞仪鉴定后建立DPSCs细胞LPS炎症模型,Western blot和Q-PCR检测各组DPSCs中MCP-1、CCR2表达,NF-κB通路激活剂TNF-α和NF-κB通路抑制剂BMS-345541作用炎症DPSCs,免疫荧光检测炎症DPSCs中P65的活化水平,划痕实验检测DPSCs迁移能力。结果:LPS诱导处理的DPSCs组显示MCP-1、CCR2基因积分光密度高于正常组,TNF-α处理DPSCs组显示MCP-1、CCR2基因积分光密度高于LPS诱导处理组和NF-κB通路抑制剂BMS-345541处理组,TNF-α处理DPSCs能促进划痕面积的愈合,且比NF-κB通路抑制剂BMS-345541处理组效果更明显。结论:在炎症状态下DPSCs表达MCP-1、CCR2基因较高,NF-κB信号途径的激活可以加强这一表达,促进DPSCs的横向迁移能力。Objective:To explore the molecular mechanism of NF-κB signaling pathway mediated by MCP-1/CCR2 axis in the migration of dental pulp stem cells(DPSCs)stemulated by inflamation factor.Methods:DPSCs of SD rats were in vitro cultured,and identified by flow cytometry.The inflammation model of DPSCs was established by LPS treatment,Western blot and Q-PCR were used to detect the expression of MCP-1 and CCR2 in DPSCs of the groups.The inflamatory DPSCs were treated by NF-κB pathway activator TNF-αand NF-κB pathway inhibitor BMS-345541 respectively,the activation of P65 in inflammatory DPSCs was detected by immunofluorescence.Scratch experiment was used to detect the migration ability of DPSCs.Results:Higher expression of MCP-1 and CCR2 genes was observed in LPS-induced DPSCs than in the controls,TNF-αtreated DPSCs showed higher expression of MCP-1 and CCR2 genes than the LPS tread and the NF-κB pathway inhibitor BMS-345541 treated cells.TNF-αtreated DPSCs showed higher scratch healing effect than LPS treated and BMS-345541 treated cells.Conclusion:The expression of MCP-1 and CCR2 genes in DPSCs is higher in inflammatory state,and the activation of NF-κB signaling pathway can enhance this expression,which can ultimately promote the migration ability of DPSCs in inflammatory state.

关 键 词：MCP-1/CCR2轴 信号途径 牙髓干细胞 迁移 

分 类 号：R781.3[医药卫生—口腔医学] R329.2[医药卫生—临床医学]