藤梨根提取物对宫颈癌Hela细胞增殖、凋亡及侵袭的影响及机制  

Effect and mechanism of ethanol extract from radix of actinidia chinensis on proliferation,apoptosis and invasion of Hela cells

作　　者：杨雨 李彤[1] 李岩[1] YANG Yu;LI Tong;LI Yan(Department of Gynecology,Central Hospital Affiliated to Shenyang Medical College,Shenyang 110024,China)

机构地区：[1]沈阳医学院附属中心医院妇科,辽宁沈阳110024

出　　处：《解剖科学进展》2024年第3期244-246,共3页Progress of Anatomical Sciences

摘　　要：目的探讨藤梨根提取物(EERAC)对宫颈癌Hela细胞增殖、凋亡及迁移的影响及分子机制。方法体外培养人宫颈癌Hela细胞,以25、50、100μg/mL的藤梨根提取物处理人宫颈癌Hela细胞,48h后终止培养,MTT方法检测细胞增殖情况;流式细胞术检测细胞凋亡情况;Transwell实验检测细胞侵袭能力;Western blot方法检测细胞细胞周期蛋白1(Cyclin D1)、周期蛋白依赖性激酶抑制因子(P21)与基质金属蛋白酶-2(MMP-2)蛋白的表达。结果不同浓度的藤梨根提取物处理后,人宫颈癌Hela细胞增殖能力降低;细胞凋亡率升高;侵袭能力降低;细胞中CyclinD1与MMP-2的表达降低,P21的表达升高,且呈浓度依赖性。结论藤梨根提取物能够抑制人宫颈癌Hela细胞增殖与侵袭,并诱导凋亡。Objective To investigate the effect and its molecular mechanism of ethanol extract from radix of actinidia chinensis(EERAC)on proliferation,apoptosis and migration of Hela cells.Methods Human cervical cancer Hela cells were cultured in vitro,treated with 25,50,100μg/mL of EERAC.After 48h,the culture was terminated,and the proliferation of Hela cells was detected by MTT method.The apoptosis of Hela cells was detected by flow cytometry.The invasion of Hela cells was detected by transwell assay.The expressions of Cyclin 1(Cyclin D1),cyclin dependent kinase inhibitor(P21)and matrix metalloproteinase-2(MMP-2)were detected by Western blot.Results After treatment with different concentrations of EERAC,the proliferation of Hela cells was decreased,the apoptosis rate was increased,the ability of invasion of Hela cells was decreased,and the expressions of CyclinD1 and MMP-2 were decreased,while the expression of P21 was increased in a concentration-dependent manner.Conclusions EERAC could inhibit the proliferation and invasion of Hela cells and induce apoptosis.

关 键 词：藤梨根提取物 HELA细胞 增殖 侵袭 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]