青檀(Pteroceltis tatarinowii)全长转录组SSR分布及其序列特征  

SSR Distribution and Sequence Characteristics of the Full-length Transcriptome of Pteroceltis tatarinowii

作　　者：郭伟 罗磊[1] 李国华[1] 王迎[1] 季霞[1] 周光锋[1] 于永畅 孙忠奎 程甜甜[1] 边晓惠 杨波[1] 王新花[1] 张林[1] Guo Wei;Luo Lei;Li Guohua;Wang Ying;Ji Xia;Zhou Guangfeng;Yu Yongchang;Sun Zhongkui;Cheng Tiantian;Bian Xiaohui;Yang Bo;Wang Xinhua;Zhang Lin(Taishan Academy of Forestry Sciences,Taian,271000;Taishan University,Taian,271021;Taian Time Garden Technology Development Co.,Ltd.,Taian,271000)

机构地区：[1]泰安市泰山林业科学研究院,泰安271000 [2]泰山学院,泰安271021 [3]泰安时代园林科技开发有限公司,泰安271000

出　　处：《分子植物育种》2024年第19期6396-6402,共7页Molecular Plant Breeding

基　　金：泰安市乡土观赏树种国家林木种质资源库项目(林场发[2016]153号);2019年山东省农业科技园区产业提升工程项目(2019YQ012);泰安市科技发展计划(引导计划)(2018NS0090)共同资助。

摘　　要：采用生物信息学方法对青檀(Pteroceltis tatarinowii)全长转录组中的SSR位点信息进行分析,以期为青檀SSR标记开发提供依据。利用MISA工具对全长转录组测序获得的基因序列进行SSR检索、位点信息分析。从11801条基因序列中检索到6524个SSR位点,平均距离为2.49 kb,发生频率为55.29%。单核苷酸所占比例为44.70%,优势重复单元是A/T,占单核苷酸的99.28%。二核苷酸所占比例为19.33%,优势重复单元是AG/TC,占二核苷酸的37.67%。大部分SSR长度集中在10~25 bp之间,所占比例为77.16%。研究结果表明青檀SSR位点类型丰富、具有较高的多态性,可为青檀SSR分子标记开发和利用、种质资源的遗传多样性分析、分子标记辅助选择育种等方面提供的理论基础。In order to provide a basis for the development of SSR markers in Pterocarpus tatarinowii,SSR loci information was analyzed in the full-length transcriptome of P.tatarinowii by bioinformatics method.MISA software was used to perform SSR loci search and information analysis in gene sequences obtained by the full-length transcriptome.A total of 6524 SSR loci distributed were detected in 11801 genes.The frequency of SSR loci was 55.29%,with an average distribution of 2.49 kb in length.In the mononucleotide repeat type accounting for 44.70%in all repeat types,the dominating motif was A/T accounting for 99.28%.In the dinucleotide repeat type accounting for 19.33%in all repeat types,the dominating motif was AG/TC accounting for 37.67%.Most of the SSR makers were 10~25 bp in length with the corresponding percentage 77.16%.The results showed that there were abundant types of SSR loci and high polymorphism in P.tatarinowii,which could provide a theoretical basis for the development and utilization of S SR molecular markers,analysis of genetic diversity of germplasm resources and molecular marker assisted breeding.

关 键 词：青檀 全长转录组 EST-SSR 

分 类 号：S792.99[农业科学—林木遗传育种]