‘热科1号’白木香内生真菌Diaporthe sp.BMX12次生代谢产物  

Secondary Metabolites of Endophytic Fungus Diaporthe sp.BMX12 from Aquilaria sinensis'Reke1'

作　　者：王启 杨理 马青云[2] 谢晴宜[2] 戴好富[2] 吴友根[1,3] 赵友兴[2] Wang Qi;Yang Li;Ma Qingyun;Xie Qingyi;Dai Haofu;Wu Yougen;Zhao Youxing(College of Horticulture,Hainan University,Haikou,570228;Haikou Key Laboratory for Research and Utilization of Tropical Natural Products,Hainan Academy of Tropical Agricultural Resource,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101;Hainan Yazhou Bay Seed Laboratory,Sanya Nanfan Research Institute of Hainan University,Sanya,572025)

机构地区：[1]海南大学园艺学院,海口570228 [2]中国热带农业科学院热带生物技术研究所,海口市热带天然产物研究与利用重点实验室,海南热带农业资源研究院,海口571101 [3]海南大学三亚南繁研究院,海南省崖州湾种子实验室,三亚572025

出　　处：《分子植物育种》2024年第19期6403-6410,共8页Molecular Plant Breeding

基　　金：海南省重大科技计划项目(ZDKJ2021031);财政部和农业农村部:国家现代农业产业技术体系项目(CARS-21);农业农村部财政专项(NFZX2021)共同资助。

摘　　要：对‘热科1号’白木香内生真菌进行分离鉴定并对其次生代谢产物进行研究。采用组织切块法分离内生真菌,通过真菌ITS序列的系统进化分析进行鉴定。采用硅胶、凝胶等柱色谱、半制备高效液相色谱等对内生真菌发酵产物的化学成分进行分离纯化,并根据核磁共振谱、质谱、圆二色谱(electronic circular dichroism,ECD)等方法鉴定化合物的结构。通过OD值法测定化合物对金黄色葡萄球菌和枯草芽孢杆菌的抑制活性。从‘热科1号’白木香中分离鉴定了内生真菌Diaporthe sp.BMX12,从其发酵产物中分离得到14个化合物,包括1个新化合物,结构分别鉴定为:diaporthone H(1)、diaporthone B(2)、diaporthone D(3)、cis-hydroxyscytalone(4)、内黄素(5)、4,6-二羟基-1(3H)-异苯并呋喃(6)、monodictyphenone(7)、diaporphasine A(8)、citreorosein(9)、4,6-dihydroxy-1H-isoindole-1,3(2H)-dione(10)、1,3,8-trihydroxyanthraquinone(11)、dicerandrol B(12)、dicerandrol A(13)、12-O-deacetyl-phomoxanthone A(14)。化合物1为聚酮类新化合物,化合物12~14对金黄色葡萄球菌ATCC 6538和枯草芽孢杆菌ATCC 6533的抑制率大于50%。To isolate the endophytic fungi from Aquilaria sinensis'Reke 1'and study the fungal secondary metabolites.The endophytic fungi were isolated by tissue cutting method,and the species were identified by phylogenetic analysis of ITS sequences.The compounds of fermentation extract of endophytic fungi were isolated and purified by silica gel and Sephadex LH-20 column chromatography and high performance liquid chromatography(HPLC).The structures of all compounds were determined by spectroscopic methods including NMR,HRESIMS and ECD.Optical density method was used to determine the compounds'inhibitory activity against Staphylococcus aureus and Bacillus subtilis.The endophytic fungus Diaporthe sp.BMX12 was isolated and identified from Aquilaria sinensis'Reke 1'.Fourteen compounds,including one new compound,were isolated from its fermentation extract and identified as diaporthone H(1),diaporthone B(2),diaporthone D(3),cis-hydroxyscytalone(4),endoc rocin(5),4,6-dihydroxy-1(3H)-isobenzofuranone(6),monodictyphenone(7),diaporphasines A(8),citreorosein(9),4,6-dihydroxy-1H-isoindole-1,3(2H)-dione(10),1,3,8-trihydroxyanthraquinone(11),dicerandrol B(12),diceran drol A(13),12-O-deacetyl-phomoxanthone A(14).Compound 1 is a new polyketide.Compounds 12~14 show the inhibition rate over 50%on S.aureus ATCC 6538 and B.subtilis ATCC 6533.

关 键 词：‘热科1号’白木香 内生真菌 次生代谢产物 聚酮类成分 抑菌活性 

分 类 号：S792.99[农业科学—林木遗传育种] Q939.9[农业科学—林学]