Spheroid construction strategies and application in 3D bioprinting  

作　　者：Chunxiang Lu Chuang Gao Hao Qiao Yi Zhang Huazhen Liu Aoxiang Jin Yuanyuan Liu 

机构地区：[1]School of Mechatronic Engineering and Automation,Shanghai University,Shanghai 200444,China [2]School of Medicine,Shanghai University,Shanghai 200444,China

出　　处：《Bio-Design and Manufacturing》2024年第5期800-818,共19页生物设计与制造（英文）

基　　金：supported by the National Natural Science Foundation of China(Nos.61973206,61703265,61803250,and 61933008);the Shanghai Science and Technology Committee Rising-Star Program(No.19QA1403700);the National Center for Translational Medicine(Shanghai)SHU Branch.

摘　　要：Tissue engineering has been striving toward designing and producing natural and functional human tissues.Cells are the fundamental building blocks of tissues.Compared with traditional two-dimensional cultured cells,cell spheres are threedimensional(3D)structures that can naturally form complex cell–cell and cell–matrix interactions.This structure is close to the natural environment of cells in living organisms.In addition to being used in disease modeling and drug screening,spheroids have significant potential in tissue regeneration.The 3D bioprinting is an advanced biofabrication technique.It accurately deposits bioinks into predesigned 3D shapes to create complex tissue structures.Although 3D bioprinting is efficient,the time required for cells to develop into complex tissue structures can be lengthy.The 3D bioprinting of spheroids significantly reduces the time required for their development into large tissues/organs during later cultivation stages by printing them with high cell density.Combining spheroid fabrication and bioprinting technology should provide a new solution to many problems in regenerative medicine.This paper systematically elaborates and analyzes the spheroid fabrication methods and 3D bioprinting strategies by introducing spheroids as building blocks.Finally,we present the primary challenges faced by spheroid fabrication and 3D bioprinting with future requirements and some recommendations.

关 键 词：SPHEROIDS STRATEGIES 3D bioprinting BIOFABRICATION 

分 类 号：TP391.73[自动化与计算机技术—计算机应用技术] R318[自动化与计算机技术—计算机科学与技术]