精氨酸对豹纹鳃棘鲈肠道发育和饲料蛋白质利用效率的影响  

The effect of arginine on intestinal development and dietary protein utilization efficiency of Plectropomus leopardus

作　　者：邓红进 梁伟兴 张佳程 迟淑艳 谭北平[1,2] DENG Hongjin;LIANG Weixing;ZHANG Jiacheng;CHI Shuyan;TAN Beiping(Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province,Zhanjiang,Guangdong Province 524088,China;College of Fisheries,Guangdong Ocean University,Zhanjiang,Guangdong Province 524088,China)

机构地区：[1]广东省水产动物精准营养与高效饲料工程技术研究中心,广东湛江524088 [2]广东海洋大学水产学院,广东湛江524088

出　　处：《中国饲料》2024年第19期90-96,共7页China Feed

基　　金：现代农业产业技术体系专项资金资助项目(CARS-47);广东省基础与应用基础研究基金项目(2021A1515010634)。

摘　　要：为研究饲料中添加不同水平精氨酸对豹纹鳃棘鲈(Plectropomus leopardus)幼鱼[初始体重(9.30±0.02)g]生长性能、体成分、肠道和肝脏组织发育的影响,在基础饲料中,分别制作精氨酸水平为2.4%(Arg2.4,对照组),2.9%(Arg2.9组),3.4%(Arg3.4组),3.9%(Arg3.9组)和4.4%(Arg4.4组)5组等氮等脂(54%粗蛋白质,9%粗脂肪)饲料。投喂豹纹鳃棘鲈幼鱼试验饲料56 d后,Arg3.4、Arg3.9和Arg4.4组蛋白质效率和蛋白质沉积率分别较Arg2.4组提高9.88%、7.41%、5.56%和17.33%、12.68%、11.99%(P<0.05)。Arg3.4、Arg3.9和Arg4.4组增重率分别较Arg2.4组提高32.03%、20.26%、17.48%(P<0.05)。相较于Arg2.4组,Arg2.9和Arg3.4组粗蛋白质含量分别提高1.62%、1.87%(P<0.05)。饲料精氨酸水平对各组鱼体水分含量、肌肉中风味氨基酸的总量均无显著影响(P>0.05)。相较于Arg2.4组,Arg3.4和Arg3.9组肥满度分别提高8.70%、8.21%(P<0.05)。精氨酸对各组间肝体比、脏体比、肝脏空泡化面积、肠道绒毛宽度和肠壁厚度均无显著影响(P>0.05)。Arg2.9、Arg3.4和Arg3.9组肠道绒毛长度和隐窝深度分别较Arg2.4组提高16.33%、20.13%、17.66%和124.36%、138.09%、110.43%(P<0.05)。基于蛋白质效率进行二元拟合后获得豹纹鳃棘鲈幼鱼饲料精氨酸适宜水平为3.52%。综上所述,精氨酸可以改善肠道组织发育,有效地促进鱼体对饲料蛋白质的利用。The purpose of this experiment was to investigate the effects of different levels of dietary arginine on growth performance,body composition,intestinal and liver tissue development in Plectropomus leopardus[initial body weight(9.30±0.02)g].In the basal diet,five feeds with isonitrogen and isolipidic(54%crude protein,9%crude lipid)were made with arginine levels 2.4%(Arg2.4,control group),2.9%(Arg2.9 group),3.4%(Arg3.4 group),3.9%(Arg3.9 group)and 4.4%(Arg4.4 group),respectively.After eight weeks feeding trial,compared with the control group,protein efficiency and protein deposition rate increased by 9.88%,7.41%,5.56%and 17.33%,12.68%,11.99%in the Arg3.4,Arg3.9 and Arg4.4 groups,respectively(P<0.05).Weight gain rate increased by 32.03%,20.26%and 17.48%in the Arg3.4,Arg3.9 and Arg4.4 groups,respectively,compared with the control group(P<0.05).Compared with the control group,the crude protein content of the Arg2.9 and Arg3.4 groups increased by 1.62%and 1.87%,respectively(P<0.05).There was no significant effect of dietary arginine levels on the moisture content of the fish and the total amount of flavor amino acids in the muscle in each group(P>0.05).Compared with the control group,condition factor increased by 8.70%and 8.21%in the Arg3.4 and Arg3.9 groups,respectively(P<0.05).No significant effect of dietary arginine was found among the groups on hepatopancreas index,viscerosomatic index,liver vacuolization area,intestinal villus width and intestinal wall thickness(P>0.05).Compared with the control group,the length of intestinal villi and depth of crypts increased by 16.33%,20.13%,17.66%and 124.36%,138.09%,110.43%in the Arg2.9,Arg3.4 and Arg3.9 groups,respectively(P<0.05).Based on protein efficiency ratio,the suitable dietary arginine level of leopard coral trout was 3.52%.In conclusion,arginine could effectively improve the development of intestinal tissues and promote fish utilization of dietary proteins.

关 键 词：精氨酸 豹纹鳃棘鲈 蛋白质利用 肠道发育 体成分 

分 类 号：S963[农业科学—水产养殖]