桃果实采后响应果生链核盘菌侵染的分子机制  

Molecular Mechanism of Postharvest Response to Monilinia fructicola Infection in Peaches

作　　者：程辰 纪艳青[1] 孙杰 冷鹏 艾合买提江[2] 刘军[2] 闵德栋 刘云国 CHENG Chen;JI Yanqing;SUN Jie;LENG Peng;Ahmtijiang;LIU Jun;MIN Dedong;LIU Yunguo(College of Life Sciences,Linyi University,Linyi 276000,Shandong,China;College of Life Science and Technology,Xinjiang University,Urumqi 830002,Xinjiang,China;Shandong(Linyi)Institute of Modern Agriculture,Zhejiang University,Linyi 276000,Shandong,China)

机构地区：[1]临沂大学生命科学学院,山东临沂276000 [2]新疆大学生命科学与技术学院,新疆乌鲁木齐830002 [3]浙江大学山东(临沂)现代农业研究院,山东临沂276000

出　　处：《食品研究与开发》2024年第19期30-36,105,共8页Food Research and Development

基　　金：山东省重点研发计划(医用食品专项计划)(2019YYSP026)。

摘　　要：褐腐病是采后桃果实最主要的侵染性病害之一,而我国桃褐腐病主要是由链核盘菌属引起,会严重降低果实品质,造成大量损失。为解析桃果实病原菌胁迫响应机制,该文采用分光光度法测定抗病相关酶活性并基于转录组学解析桃果实响应果生链核盘菌的分子机制。结果发现,桃果实的过氧化物酶、苯丙氨酸解氨酶和β-1,3-葡聚糖酶在侵染的不同时期启动了响应反应,参与了桃果实抵御果生链核盘菌侵染过程。基因本体(gene ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析表明,桃果实对果生链核盘菌的侵染会产生复杂的防御反应。Brown rot is one of the main infectious diseases in postharvest peaches.In China,brown rot of peaches is mainly caused by Monilinia fructicola,which can seriously reduce fruit quality and cause signifi-cant losses.To analyze the response mechanism of pathogen stress of peaches,spectrophotometry was used to measure the activity of disease-resistant enzymes and explore the molecular mechanism of peach response to Monilinia fructicola based on transcriptomics.It was found that the peroxidase(POD),phenylalanine ammo-nia lyase(PAL),andβ-1,3-glucanase(GLU)in peach initiated response reactions at different stages of in-fection,participating in the resistance of peach to the infection of Monilinia fructicola.Moreover,Gene ontol-ogy(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis indicated that peaches could exhibit complex defense responses against the infection of Monilinia fructicola.

关 键 词：果生链核盘菌 转录组 防御酶 防御反应 真菌病害 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程] S436.621[轻工技术与工程—食品科学与工程]