CCDC181 is required for sperm flagellum biogenesis and male fertility in mice  

作　　者：Xiang-Jun Zhang Xiao-Ning Hou Jian-Teng Zhou Bao-Lu Shi Jing-Wei Ye Meng-Lei Yang Xiao-Hua Jiang Bo Xu Li-Min Wu Qing-Hua Shi 

机构地区：[1]Division of Reproduction and Genetics,First Affiliated Hospital of University of Science and Technology of China(USTC),Hefei National Laboratory for Physical Sciences at Microscale,School of Basic Medical Sciences,Division of Life Sciences and Medicine,Biomedical Sciences and Health Laboratory of Anhui Province,Institute of Health and Medicine,Hefei Comprehensive National Science Center,University of Science and Technology of China,Hefei,Anhui 230001,China

出　　处：《Zoological Research》2024年第5期1061-1072,共12页动物学研究（英文）

基　　金：supported by the National Natural Science Foundation of China(82071709,81971446,82171599,82374212);Global Select Project(DJK-LX-2022010)of the Institute of Health and Medicine,Hefei Comprehensive National Science Center;Joint Fund for New Medicine of USTC(YD9100002034)。

摘　　要：The structural integrity of the sperm flagellum is essential for proper sperm function.Flagellar defects can result in male infertility,yet the precise mechanisms underlying this relationship are not fully understood.CCDC181,a coiled-coil domain-containing protein,is known to localize on sperm flagella and at the basal regions of motile cilia.Despite this knowledge,the specific functions of CCDC181 in flagellum biogenesis remain unclear.In this study,Ccdc181 knockout mice were generated.The absence of CCDC181 led to defective sperm head shaping and flagellum formation.Furthermore,the Ccdc181 knockout mice exhibited extremely low sperm counts,grossly aberrant sperm morphologies,markedly diminished sperm motility,and typical multiple morphological abnormalities of the flagella(MMAF).Additionally,an interaction between CCDC181 and the MMAF-related protein LRRC46 was identified,with CCDC181 regulating the localization of LRRC46 within sperm flagella.These findings suggest that CCDC181 plays a crucial role in both manchette formation and sperm flagellum biogenesis.

关 键 词：Male infertility CCDC181 MMAF SPERMIOGENESIS Flagellum biogenesis 

分 类 号：Q954.4[生物学—动物学]