野尻链霉菌9-13对大豆炭疽病病原菌的抑制活性及作用机制  

Antifungal activity and action mechanism of Streptomyces nojiriensis 9-13 againstfungal pathogens associated with soybean anthracnose

作　　者：石妞妞[1,2] 连金番[3] 邱德柱 居骋 张楠 殷越 李本金[1,2] 杜宜新[1,2] Shi Niuniu;Lian Jinpan;Qiu Dezhu;Ju Cheng;Zhang Nan;Yin Yue;Li Benjin;Du Yixin(Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou 350013,Fujian Province,China;Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou 350001,Fujian Province,China;Institute of Crop Research,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,750002,Ningxia Hui Autonomous Region,China;College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350000,Fujian Province,China;Institute for the Control of Agrochemicals,Beijing 100125,China)

机构地区：[1]福建省农业科学院植物保护研究所,福州350013 [2]福建省农业科学院植物保护研究所,福建省作物有害生物监测与治理重点实验室,福州350001 [3]宁夏农林科学院农作物研究所,银川750002 [4]福建农林大学植物保护学院,福州350000 [5]农业农村部农药检定所,北京100125

出　　处：《植物保护学报》2024年第4期830-840,共11页Journal of Plant Protection

基　　金：福建省属公益项目(2023R1022004);福建省自然科学基金(2021J01476);福建省人民政府中国农业科学院农业高质量发展超越“5511”协同创新工程(XTCXGC2021017,XTCXGC2021011);福建省农业科学院农作物重大有害生物灾变机制与绿色防控科技创新团队(CXTD2021002-1)。

摘　　要：为明确野尻链霉菌Streptomyces nojiriensis 9-13对大豆炭疽病病原菌的抑制活性及作用机制,采用对峙培养法、菌丝生长速率法和孢子萌发法分别测定该菌株对引起大豆炭疽病的平头炭疽菌Colletotrichum truncatum、胶孢炭疽菌C. gloeosporioides和C. plurivorum,以及对苯醚甲环唑和啶氧菌酯的平头炭疽菌抗药性突变体的拮抗活性、抑菌活性和对分生孢子萌发的抑制活性;观察野尻链霉菌9-13发酵液对平头炭疽菌菌丝形态和细胞结构的影响。结果表明:野尻链霉菌9-13对引起大豆炭疽病的3种病原菌平头炭疽菌、胶孢炭疽菌和C. plurivorum均具有较强的拮抗作用。野尻链霉菌9-13的10%发酵液对3种大豆炭疽菌菌丝生长的抑制率为56.67%~87.04%,且抑制活性与发酵液的浓度呈正相关。此外,野尻链霉菌9-13对苯醚甲环唑和啶氧菌酯的平头炭疽菌抗药性突变体同样具有较强的拮抗作用。野尻链霉菌9-13的10%发酵液对苯醚甲环唑和啶氧菌酯的平头炭疽菌抗药性突变体的菌丝生长抑制率分别为74.79%~77.80%和46.53%~58.33%。野尻链霉菌9-13的10%发酵液对9种供试炭疽菌分生孢子萌发的抑制率均达100.00%。野尻链霉菌9-13发酵液可以破坏平头炭疽菌菌丝的细胞膜结构,使细胞发生质壁分离。发酵液处理后平头炭疽菌菌丝表现出膜相关的红色荧光斑点。表明野尻链霉菌9-13是一株具有开发前景的生防菌株。In order to uncover the antifungal activity and action mechanism of Streptomyces nojiriensis 9-13 against soybean anthracnose caused by Colletotrichum species,antifungal effects against different Colletotrichum spp.,including C.truncatum,C.gloeosporioides,C.plurivorum,difenoconazole-resistant mutants and picoxystrobin-resistant mutants of C.truncatum,were assessed using the method of dual culture and the suppression of mycelial growth and conidial germination.In addition,the effects of S.nojiriensis 9-13 on the mycelial morphology and cellular structure of C.truncatum were studied with scanning electron microscopy,transmission electron microscopy and fluorescence confocal microscopy.The results showed that the strain 9-13 presented significant antagonistic activity against three pathogens associated with soybean anthracnose,e.g.C.truncatum,C.gloeosporioides,and C.plurivorum.The inhibition rate of 10%fermentation filtrate of the strain 9-13 against the above three Colletotrichum species ranged from 56.67%-87.04%and increased in a dose-dependent manner.Additionally,the strain 9-13 also exhibited strong antagonistic activity against difenoconazole-resistant mutants and picoxystrobin-resistant mutants of C.truncatum.The inhibition rate of 10%fermentation filtrate of the strain 9-13 against difenoconazole-resistant mutants and picoxystrobin-resistant mutants was 74.79%-77.80%and 46.53%-58.33%,respectively.And 10%fermentation filtrate of the strain 9-13 could completely inhibit spore germination of all tested pathogens.Scanning and transmission electron microscopy revealed that strain 9-13 fermentation could induce severe mycelial deformation,disrupt cell membrane integrity and cause cell plasmolysis.In addition,hyphae treated with strain 9-13 fermentation exhibited membrane-associated red fluorescence puncta upon staining with FM4-64 compared with the control group.These results indicate that S.nojiriensis 9-13 is a promising biocontrol agent.

关 键 词：大豆炭疽病 野尻链霉菌 抑菌机理 生物防治 抗性治理 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]