D-AP5对PD小鼠黑质多巴胺能神经元簇状放电影响  

Effect of intracerebroventricular injection of D-AP5 on burst firing of dopaminergic neurons in the substantia nigra in a mouse model of Parkinson’s disease

作　　者：赵继虎 刘恒 孙鹏[1] ZHAO Jihu;LIU Heng;SUN Peng(Department of Neurosurgery,The Affiliated Hospital of Qingdao University,Qingdao 266555,China)

机构地区：[1]青岛大学附属医院神经外科,山东青岛266555 [2]青岛大学护理学院

出　　处：《青岛大学学报（医学版）》2024年第4期487-490,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金青年科学基金项目(32000795);中国博士后基金面上项目(2017M622129);山东省自然科学基金青年科学基金项目(ZR2022MH022,ZR2020QC095)。

摘　　要：目的探究侧脑室注射D(-)-2-氨基-5-磷戊酸(D-AP5)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)帕金森病(PD)模型小鼠黑质区多巴胺能神经元簇状放电的影响。方法将小鼠随机分为对照组、D-AP5组、MPTP组和D-AP5+MPTP组。采用电生理实验记录小鼠黑质区多巴胺能神经元的簇状放电百分比。结果析因设计方差分析显示,D-AP5和MPTP两者存在交互作用(FMPTP=4.601,P<0.05;FD-AP5=2.399,P>0.05;FMPTP×D-AP5=12.020,P<0.01)。单独效应分析显示,在未注射MPTP的两组小鼠中,D-AP5组小鼠黑质区多巴胺能神经元簇状放电百分比与对照组小鼠相比差异无显著性(F=1.916,P>0.05);在注射MPTP的两组小鼠中,D-AP5+MPTP组小鼠黑质区多巴胺能神经元簇状放电百分比较MPTP组明显降低(F=12.094,P<0.01)。结论侧脑室注射D-AP5可降低MPTP模型小鼠黑质区多巴胺能神经元簇状放电百分比。Objective To investigate the effect of intracerebroventricular injection of D(-)-2-amino-5-phosphonopentanoic acid(D-AP5)on the burst firing of dopaminergic neurons in the substantia nigra ina mouse model of Parkinson’s disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods Mice were randomly divided into control group,D-AP5 group,MPTP group,and D-AP5+MPTP group.The bursting rate of substantia nigra dopaminergic neurons was recorded through the electrophysiological test.Results The factorial analysis of variance indicated an interaction between D-AP5 and MPTP(F MPTP=4.601,P<0.05;F D-AP5=2.399,P>0.05;F MPTP×D-AP5=12.020,P<0.01).The individual effect analysis showed that there was no significant difference in the bursting rate of substantia nigradopaminergic neurons between the D-AP5 group and the control mice(F=1.916,P>0.05);and the D-AP5+MPTP group showed a significantly lower bursting rate compared with the MPTP group(F=12.094,P<0.01).Conclusion Intracerebroventricular injection of D-AP5 can reduce the bursting rate of dopaminergic neurons in the substantia nigra of MPTP model mice.

关 键 词：帕金森病 受体 N-甲基-D-天冬氨酸 兴奋性氨基酸拮抗剂 黑质 多巴胺能神经元 电生理学 小鼠 近交C57BL 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R392.11[医药卫生—临床医学]