适用于大鼠气道高压脉冲电场消融的导管电极研制和应用  

Development and Application of Catheter Electrodes for Rat Airway High-Voltage Pulsed Electric Field Ablation

作　　者：张娜娜 安怡蓉 田嘉玮 韩轩 屈申奥 冷昊泽 陶诗然 任冯刚[1,2,3,4] 吕毅 朱皓阳[2,3,5] ZHANG Nana;AN Yirong;TIAN Jiawei;HAN Xuan;QU Shen’ao;LENG Haoze;TAO Shiran;REN Fenggang;LYU Yi;ZHU Haoyang(Institute of Regenerative and Reconstructive Medicine,Med-X Institute,First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,710049;National Local Joint Engineering Research Center for Precision Surgery&Regenerative Medicine,First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,710061;Shaanxi Provincial Key Laboratory of Magnetic Medicine,Xi'an,710061;Department of Hepatobiliary Surgery,First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,710061;Department of Anesthesiology,First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,710061)

机构地区：[1]西安交通大学第一附属医院,Med-X研究院再生与重建医学研究所,西安市710049 [2]西安交通大学第一附属医院,精准外科与再生医学国家地方联合工程研究中心,西安市710061 [3]陕西省磁医学重点实验室,西安市710061 [4]西安交通大学第一附属医院肝胆外科,西安市710061 [5]西安交通大学第一附属医院麻醉手术部,西安市710061

出　　处：《中国医疗器械杂志》2024年第5期568-572,共5页Chinese Journal of Medical Instrumentation

基　　金：国家重点研发计划资助(2023YFF0713700);国家自然科学基金青年项目(82202297);西安交通大学第一附属医院科研发展基金(2021QN-23)。

摘　　要：高压脉冲电场消融技术在慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)的临床治疗中展现出广阔的应用前景。然而,该技术目前仅在小样本的临床病例中进行了初步应用,其具体治疗机制尚未阐明。为了加速其临床应用进程,迫切需要通过大量动物实验来探究相关分子机制。大鼠相较于其他实验动物,更易于构建COPD模型,并且拥有相对完备的组织和分子学实验试剂是进行探究脉冲电场消融COPD机制研究的理想模型。因此,开发适用于SD大鼠呼吸道的消融导管电极尤为重要。该研究首先观察了大鼠的气道解剖结构,并对导管的材质、硬度、直径、电极环尺寸、正负极间距、导管的绝缘处理、导丝与电极环的焊接技术,以及导管通气孔等关键设备参数进行了深入调研。在此基础上,制备了一种带有通气功能的PVC消融电极导管。接下来,利用COMSOL软件模拟了该电极可施加的场强,并在正常大鼠中进行了验证,以评估电极的电学安全性和有效性。这些研究成果为后续高压脉冲电场治疗COPD的相关机制研究奠定了实践基础。High-voltage pulsed electric field(HV-PEF)ablation technology has demonstrated promising applications in the clinical treatment of chronic obstructive pulmonary disease(COPD).However,its use has been limited to exploratory applications in a small number of cases,and the underlying mechanisms remain largely undefined.To facilitate broader clinical implementation,comprehensive molecular mechanism studies via extensive animal experimentation are essential.Rats,due to their ease of modeling COPD and the availability of comprehensive molecular reagents,serve as an optimal model for such studies.Consequently,the development of electrodes specifically designed for HV-PEF respiratory ablation in SD rats is of significant importance.In this study,we meticulously examined the anatomical structure of rat airways and investigated various equipment parameters,including material composition,rigidity,diameter,electrode ring dimensions,spacing between positive and negative poles,insulation coating for the catheters,welding techniques between the guidewire and electrode ring,and the design of vent holes in the catheter.Based on these considerations,we fabricated PVC ablation electrode catheters with integrated ventilation functionality.Subsequently,we employed finite element simulation to estimate the field strengths that could be applied by these electrodes.The simulation results were then validated in normal rats to assess the electrical safety and efficacy of the electrodes.These findings laid the groundwork for further investigation into the mechanisms of HV-PEF treatment for COPD.

关 键 词：高压脉冲电场 导管电极 气道消融 慢性阻塞性肺疾病 

分 类 号：R197.39[医药卫生—卫生事业管理] TH77[医药卫生—公共卫生与预防医学]