Age-Related Alterations in Peripheral Immune Landscape with Magnified Impact on Post-Stroke Brain  

作　　者：Jianan Lu Huaming Li Guoqiang Zhangi Fan Yangi Xiaotao Zhangi An Pingi Zhouhan Xui Yichen Gui Rui Wangi Dan Yingi Jianjian Liui Jianmin Zhangi Ligen Shi 

机构地区：[1]Department of Neurosurgery,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,Zhejiang,China [2]Clinical Research Center for Neurological Diseases of Zhejiang Province,Hangzhou,China.

出　　处：《Research》2024年第3期51-69,共19页研究（英文）

基　　金：grants from the Natural Science Foundation of Zhejiang Province(LQ21H250001);the National Natural Science Foundation of China(82322022 and 82001460).

摘　　要：Immunosenescence refers to the multifaceted and profound alterations in the immune system brought about by aging,exerting complex influences on the pathophysiological processes of diseases that manifest upon it.Using a combination of single-cell RNA sequencing,cytometry by time of flight,and various immunological assays,we investigated the characteristics of immunosenescence in the peripheral blood of aged mice and its impact on the cerebral immune environment after ischemic stroke.Our results revealed some features of immunosenescence.We observed an increase in neutrophil counts,concurrent with accelerated neutrophil aging,characterized by altered expression of aging-associated markers like CD62L and consequential changes in neutrophil-mediated immune functions.Monocytes/macrophages in aged mice exhibited enhanced antigen-presentation capabilities.T cell profiles shifted from naive to effector or memory states,with a specific rise in T helper 1 cells and T helper 17 cells subpopulations and increased regulatory T cell activation in CD4 T cells.Furthermore,regulatory CD8 T cells marked by Klra decreased with aging,while a subpopulation of exhausted-like CD8 T cells expanded,retaining potent immunostimulatory and proinflammatory functions.Critically,these inherent disparities not only persisted but were further amplified within the ischemic hemispheres following stroke.In summary,our comprehensive insights into the key attributes of peripheral immunosenescence provide a vital theoretical foundation for understanding not only ischemic strokes but also other age-associated diseases.

关 键 词：alterations retaining IMPACT 

分 类 号：R743.3[医药卫生—神经病学与精神病学]