青海草原毛虫成虫触角感器观察及GqinOBPs表达分析  

Observations on the ultrastructure of adult Gynaephora qinghaiensis antennal sensilla and tissue expression analysis of GqinOBPs

作　　者：南彦斌 刘占玲 寇桂香 王克鑫 李蓉蓉 周渊涛 NAN Yanbin;LIU Zhanling;KOU Guixiang;WANG Kexin;LI Rongrong;ZHOU Yuantao(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农牧学院,西宁810016

出　　处：《植物保护》2024年第5期15-25,71,共12页Plant Protection

基　　金：青海省科技厅青年基金(2022-ZJ-949Q)。

摘　　要：为明确青海草原毛虫Gynaephora qinghaiensis触角感受器的类型、分布及GqinOBPs基因在嗅觉识别过程中的功能,本研究利用扫描电镜对其触角进行观察,并通过RT-qPCR测定GqinOBPs基因在雌雄成虫组织中的表达情况。在青海草原毛虫雌雄成虫触角上发现分泌孔及8种感器,包括刺形感器、毛形感器、Bohm氏鬃毛、单孔栓感器、鳞形感器、锥形感器、柱形感器和腔锥形感器。其中刺形感器、毛形感器、Bohm氏鬃毛和锥形感器分别有6、3、2和3种亚型。GqinOBPs的组织表达结果显示,GqinOBP 6、GqinOBP 8、GqinOBP 9和GqinOBP 16在雄成虫触角中的相对表达量显著高于其他组织,GqinOBP 4、GqinOBP 10、GqinOBP 13~GqinOBP 15和GqinOBP 17在雄成虫翅中的相对表达量最高。GqinOBP 2、GqinOBP 12和GqinOBP 17在雌虫组织中的整体表达情况较好且GqinOBP17的表达具雌性偏好(P<0.05)。本研究为深入了解青海草原毛虫化学感受机制奠定基础,同时也为寻找青海草原毛虫绿色防治的分子靶标提供理论依据。To determine the types and distribution of antennal sensilla and the function of GqinOBP genes in the process of olfactory recognition,adult antennae of Gynaephora qinghaiensis were observed using a scanning electron microscope.The expression of GqinOBP genes in the tissues of female and male adults was determined by RT-qPCR.The antennae of G.qinghaiensis exhibited smell pores and eight types of sensilla:sensilla chaetica,sensilla trichodea,Bohm’s bristles,uniporous peg sensilla,sensilla squamous,sensilla basiconica,sensilla cylindric,and sensilla coeloconica.Notably,sensilla chaetica,sensilla trichodea,Bohm’s bristles,and sensilla basiconica had six,three,two,and three subtypes,respectively.Tissue expression analysis of GqinOBPs showed that the relative expression levels of GqinOBP 6,GqinOBP 8,GqinOBP 9,and GqinOBP 16 in the antennae of male adults were significantly higher than in other tissues.In contrast,GqinOBP 4,GqinOBP 10,GqinOBP 13 to GqinOBP 15,and GqinOBP 17 showed highest expression levels in the wings of male adults.Furthermore,the expressions of GqinOBP 2,GqinOBP 12,and GqinOBP 17 were higher in female tissues,with GqinOBP 17 showing a preference for females(P<0.05).This study provides foundational insights into the chemosensory mechanisms of G.qinghaiensis and offers a theoretical basis for finding molecular targets for the green control of G.qinghaiensis.

关 键 词：青海草原毛虫 触角感器 扫描电镜 气味结合蛋白 组织表达 

分 类 号：S8122[农业科学—草业科学]