碱性蛋白酶的皮肤接触损伤和直接饲喂对胃损伤研究  

Study on Skin Contact Damage and Stomach Injury of Direct Feeding of Alkaline Protease

作　　者：宣丽 权志中 XUAN Li;QUAN Zhizhong(Shenyang Academy of Agricultural Sciences,Liaoning Shenyang 110091,China;Liaoning Complete Biotechnology Co.,Ltd.,Liaoning Tieling 112600,China)

机构地区：[1]沈阳市农业科学院,辽宁沈阳110091 [2]辽宁康普利德生物科技有限公司,辽宁铁岭112600

出　　处：《饲料工业》2024年第19期99-106,共8页Feed Industry

基　　金：国家重点研发计划资助项目[2023YFD1301801]。

摘　　要：试验旨在研究碱性蛋白酶对皮肤的接触损伤和直接饲喂对胃的损伤,以豚鼠为动物模型,碱性蛋白酶按0.02、0.05、0.10、0.20 g/mL连续接触豚鼠皮肤7 d,按0、1、2、5 g/100 g连续拌料饲喂健康豚鼠3 d,观察碱性蛋白酶对皮肤和胃的损伤情况。结果显示:(1)碱性蛋白酶连续接触皮肤3 d,不同浓度组均造成较为明显的皮肤损伤和结痂,损伤面积(>40%)差异不显著;当接触天数增加到7 d时,0.10、0.20 g/mL浓度组造成的损伤面积(>90%)显著高于0.02、0.05 g/mL浓度组(=79%)。(2)0.02、0.10、0.20 g/mL碱性蛋白酶酶液连续接触皮肤2 d,皮肤损伤评分即显著增加;当接触到7 d时,各浓度组评分均>3,达到严重溃烂的程度。(3)用碱性蛋白酶喂食健康豚鼠3 d,当添加量为1 g/100 g时,豚鼠胃表观出现损伤,但没有死亡;当添加量为2 g/100 g时,豚鼠胃壁明显变薄,皱襞减少,死亡率为25%;当添加量为5 g/100 g时,豚鼠的胃壁变得极薄,死亡率达到75%。结果表明:0.02~0.20 g/mL碱性蛋白酶酶液连续接触皮肤7 d,即可造成皮肤的严重损伤;在饲料中添加高剂量碱性蛋白酶可造成胃的表观损伤,当添加量>2 g/100 g时可造成动物死亡。The experiment was conducted to study the skin contact damage and stomach injury of direct feeding of alkaline protease.Guinea pigs were taken as animal models,the damage of the skin and stom⁃ach caused by alkaline protease were observed with alkaline protease at 0.02,0.05,0.10,0.20 g/mL for skin contact 7 days,and 0,1,2,5 g of alkaline protease were mixed into 100 g feed then fed to healthy guinea pigs for 3 days.The results showed that:①the continuous contact of alkaline protease with skin 3 days,different concentration groups were caused obvious skin damage and scab,but the damage area(>40%)were not significantly different;when the contact days increased to 7 days,the damage area of 0.10,0.20 g/mL concentration groups(>90%)were significantly higher than that of 0.02,0.05 g/mL concentration groups(=79%).②When the alkaline protease solution of 0.02,0.10,0.20 g/mL were con⁃tinuously exposed to the skin for 2 days,the skin injury score increased significantly;when contact skin for 7 days,the score of each concentration group was>3,reaching the degree of severe ulceration.③Healthy guinea pigs were fed with alkaline protease for 3 days,when the dosage was 1 g/100 g,the guinea pigs'stomach was damaged,but there was no death;when the dosage was 2 g/100 g,the stomach walls of guinea pigs were thinner and the folds were reduced,the mortality rate was 25%;when the dosage was 5 g/100 g,the stomach walls of guinea pigs were extremely thin and the mortality rate reached 75%.The results indicated that:0.02-0.20 g/mL of alkaline protease enzyme liq⁃uid could cause serious damage when it was continuously exposed to the skin for 7 days;the addition of high dose of alkaline protease in the feed could cause stomach injury,and when the additive amount was>2 g/100 g,it could cause animal death.

关 键 词：碱性蛋白酶 致敏性 皮肤接触损伤 胃损伤 安全饲喂 

分 类 号：S816.79[农业科学—饲料科学]