Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene  

作　　者：Yucong Gong Xiangli Li Daqing Ma Lai Wang Lin Zhou Caiwei Lu Yi Xiao Xinfu Zhang 

机构地区：[1]State Key Laboratory of Fine Chemicals,Frontiers Science Center for Smart Materials Oriented Chemical Engineering,Dalian University of Technology,Dalian 116024,China [2]China Academy of Safety Science and Technology,Beijing 100012,China

出　　处：《Chinese Journal of Chemical Engineering》2024年第8期220-225,共6页中国化学工程学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(22278059,22174009,and 22078047);Fundamental Research Funds for the Central Universities(DUT22LAB601 and DUT22LAB608)。

摘　　要：The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells.However,most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time.Herein,Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time.This tracker is composed of a lipophilic boron-dipyrromethene(BODIPY)derivative and a hydrophilic zwitterion to form an amphiphilic structure,which enables its targeting ability toward cell membranes.Moreover,a reactive ester group is included to bind with proteins through covalent bonds in cell membranes nonspecifically,which extends retention time in cell membranes.Mem580-F-Sulfo shows intense brightness(94600),with a high molar absorption coefficient of up to about 100000 L·mol^(-1)·cm^(-1)and a fluorescence quantum yield of up to 0.97.It shows fast cell membrane targeting ability and long retention up to 90 min.In brief,this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.

关 键 词：Plasma membrane PROTEIN TRACKER IMAGING MICROSTRUCTURE 

分 类 号：Q2-33[生物学—细胞生物学] O657.3[理学—分析化学]