刺山柑果风湿止痛凝胶膏对SD大鼠胚胎-胎仔发育的影响  

Effect of Capparis spinosa fruits pain gel paste on embryo-fetal development of SD rats

作　　者：许文黎[1] 李曙芳[1] 满尓巴哈·海如拉 王新钢[1] 程波 刘欢[1] 孙鸽[1] 袁慧[1] 杨伟俊 XU Wenli;LI Shufang;MANERBAHA·Hairula;WANG Xingang;CHENG Bo;LIU Huan;SUN Ge;YUAN Hui;YANG Weijun(China Institute for Radiation Protection,Shanxi Key Laboratory of Drug Toxicology and Preclinical Study of Radiopharmaceuticals/CNNC Key Laboratory on Radiotoxicology and Radiopharmaceutical Preclinical Evaluation,Taiyuan 030006,Shanxi;Institute of Materia Medica of Xinjiang,Urumqi 830004,Xinjiang,China)

机构地区：[1]中国辐射防护研究院,药物毒理及放射性药物临床前研究山西省重点实验室/中核放射毒理与放射性药物临床前评价重点实验室,山西太原030006 [2]新疆维吾尔自治区药物研究所,新疆乌鲁木齐830004

出　　处：《癌变．畸变．突变》2024年第5期399-404,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01A164)。

摘　　要：目的:观察刺山柑果风湿止痛凝胶膏对SD大鼠胚胎-胎仔发育的影响。方法:实验设刺山柑果风湿止痛凝胶膏高(117.8 g/m^(2))、中(58.9 g/m^(2))、低(29.5 g/m^(2))剂量组,辅料对照组(辅料帖)和阳性对照组(环磷酰胺),妊娠第6天(GD6)~GD15每天经皮给药1次,阳性对照组于GD12皮下注射环磷酰胺。实验期间观察孕鼠的一般状况、体质量和饲料消耗量;GD20处死孕鼠,取胎仔,检查子宫连胎质量、黄体数、着床数、胎仔质量、胎仔身长、胎仔尾长、活胎数、死胎数、吸收胎数、胎仔性别及外观、内脏和骨骼畸形。结果:刺山柑果风湿止痛凝胶膏各剂量组孕鼠体质量、体质量增量、摄食量、子宫连胎质量、子宫和卵巢的脏器质量及其系数、平均黄体数、着床前丢失率、平均着床数、着床率、平均活胎数、活胎率、死胎率、吸收胎率、胎仔性别比、胎仔的外观异常率、窝均体质量、窝均身长、窝均尾长、骨骼畸形率和内脏畸形率与辅料对照组相比差异均无统计学意义(均为P>0.05)。结论:刺山柑果风湿止痛凝胶膏对SD大鼠胚胎-胎仔发育毒性的未见不良反应水平为117.8 g/m^(2),是临床拟用体表剂量的12倍。在本试验条件下,SD大鼠经皮给予刺山柑果风湿止痛凝胶膏对胚胎-胎仔发育无毒性影响。OBJECTIVE:To investigate effects of the Capparis spinosa fruit rheumatism pain relief gel on embryo-fetal development in SD rats.METHODS:Rats were divided into Capparis spinosa fruit rheumatism pain relief gel high(117.8 g/m^(2)),medium(58.9 g/m^(2)),low(29.5 g/m^(2))dose groups,excipient control group(excipient paste)and positive control group(cyclophosphamide).The drug was given once a day from the 6th to 15th days after pregnancy,and the positive control group was injected subcutaneously with cyclophosphamide on GD12.The general condition,body weight and feed consumption of pregnant rats were observed during the experiment.The pregnant rats were sacrificed on GD20,and the fetuses were taken to check the uterine combined fetal weight,luteal number,implantation number,fetal weight,fetal body length,fetal tail length,live birth number,dead birth number,absorbed birth number,fetal gender and appearance,visceral and skeletal malformation.RESULTS:There were no significant differences in body weight,body weight gain,food intake,uterine combined fetal weight,uterine and ovarian organ weight and its coefficient,average luteal number,loss rate before implantation,average implantation number,implantation rate,average live birth number,live birth rate,dead birth rate,absorbed birth rate,fetal gender ratio,fetal appearance abnormality rate,litter weight,litter body length,litter tail length,skeletal malformation rate and visceral malformation rate between the pregnant rats in the different dose groups of Capparis spinosa fruit rheumatism pain relief gel and the excipient control group(all P>0.05).CONCLUSION:The no-adverse reaction level of capsicum frutescens rheumatism pain relieving gel on embryo-fetal development of SD rats was 117.8 g/m^(2),which was 12 times of the body surface dose to be used in clinic.Under the conditions of this study,transdermal administration of capsicum frutescens rheumatism pain relieving gel on embryo-fetal development of SD rats had no toxic effect.

关 键 词：刺山柑果风湿止痛凝胶膏 SD大鼠 胚胎-胎仔发育 生殖毒性 

分 类 号：Q355[生物学—遗传学] R394.6[医药卫生—医学遗传学]