lncRNA EBLN3P调节miR-340-5p/线粒体转录因子A轴对非小细胞肺癌细胞恶性生物学行为的影响  

Impact of lncRNA EBLN3P on the malignant biological behavior of non small cell lung cancer cells by regulating miR-340-5p/mitochondrial transcription factor A axis

作　　者：陈燕 蔺淑娟 Chen Yan;Lin Shujuan(Department of Thoracic and Cardiovascular Surgery,the Third People's Hospital of Hubei Province,Wuhan 433033,China)

机构地区：[1]湖北省第三人民医院胸心血管外科,武汉433033

出　　处：《解剖学杂志》2024年第4期303-307,320,共6页Chinese Journal of Anatomy

摘　　要：目的:探讨长链非编码RNA EBLN3P调节微小RNA-340-5p(miR-340-5p)/线粒体转录因子A(TFAM)轴对非小细胞肺癌(NSCLC)细胞恶性生物学行为的影响及其机制。方法:使用NSCLC细胞系为研究对象。将A549细胞分为Ctrl组、si-NC组、si-EBLN3P组、si-EBLN3P+NC inhibitor组、si-EBLN3P+miR-340-5p inhibitor组,应用MTT、EdU、Transwell检测细胞增殖、迁移、侵袭;双荧光素酶报告基因实验验证EBLN3P、miR-340-5p、TFAM 3者间的靶向关系;分别检测TFAM、miR-340-5p、E-cadherin、N-cadherin蛋白表达。结果:EBLN3P在肺癌组织和细胞中表达上调;在NSCLC细胞中,TFAM表达上调,miR-340-5p表达降低。抑制EBLN3P表达可显著抑制NSCLC细胞增殖、迁移与侵袭,且E-cadherin蛋白表达上升,N-cadherin蛋白表达下降。EBLN3P、TFAM与miR-340-5p存在靶向关系。结论:EBLN3P在NSCLC组织与细胞中呈高表达,抑制EBLN3P表达可通过调节miR-340-5p/TFAM信号轴,抑制NSCLC细胞增殖、迁移与侵袭。Objective:To investigate the impact and mechanism of long non-coding RNA EBLN3P on the malignant biological behavior of non small cell lung cancer(NSCLC)cells by regulating microRNA-340-5p(miR-340-5p)/mitochondrial transcription factor A(TFAM)axis.Methods:NSCLC cell lines were applied as research objects.A549 cells were grouped into ctrl group,si-NC group,si-EBLN3P group,si-EBLN3P+NC inhibitor group,and si-EBLN3P+miR-340-5p inhibitor group.Cell proliferation,migration,and invasion were detected by MTT,EdU,Transwell respectively.The targeting relationship among EBLN3P,miR-340-5p,and TFAM was verified by dual luciferase reporter assay,and expression of TFAM,miR-340-5p,E-cadherin,and N-cadherin proteins was detected by qRT-PCR and Western blotting respectively.Results:The expression of EBLN3P was up-regulated in lung cancer tissues and cells.In NSCLC cells,the expression of TFAM was up-regulated and the expression of miR-340-5p was decreased.Inhibiting the expression of EBLN3P could obviously inhibit the proliferation,migration,and invasion of NSCLC cells,and the expression of E-cadherin protein increased,while the expression of N-cadherin protein decreased.There was a targeting relationship between EBLN3P,TFAM and miR-340-5p.Conclusion:EBLN3P is highly expressed in NSCLC tissues and cells,inhibiting the expression of EBLN3P can inhibit the proliferation,migration,and invasion of non small cell lung cancer cells by regulating the miR-340-5p/TFAM signal axis.

关 键 词：长链非编码RNA EBLN3P 微小RNA-340-5p 线粒体转录因子A 非小细胞肺癌 增殖 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]