桑叶中1-脱氧野尻霉素的提取及含量对比研究  

Extraction and content comparison of 1-deoxynojirimycin from mulberry leaves

作　　者：王艳萍[1] 曹莹 WANG Yanping;CAO Ying(Department of Life Science,Yun Cheng University,Shanxi Yuncheng 044000,China)

机构地区：[1]运城学院生命科学系,山西运城044000

出　　处：《食品工程》2024年第3期93-96,共4页Food Engineering

基　　金：运城学院食品科学与工程重点学科项目(XK-2021008);运城学院特色农产品发展学科群科研项目(SKX-202211)。

摘　　要：研究了桑叶中1-脱氧野尻霉素(DNJ)的提取条件,并采用高效液相色谱-紫外检测法测定前期研究筛选出的9种运城市优势桑树品种中的1-脱氧野尻霉素(DNJ)含量。以提取温度、提取功率、提取时间为影响因素,采用单因素试验确定各因素最优水平,并在此基础上进行三因素三水平正交试验优化提取条件,得出最佳提取条件为:提取温度70℃,提取功率350 W,提取时间60 min。在此提取条件下峰分离完好,无拖尾、无连续;1-脱氧野尻霉素在0.1~1.5 mg/mL质量浓度范围内,线性关系良好(R^(2)=0.9989);测得不同品种桑叶中1-脱氧野尻霉素含量有差异,赤红大叶(8-12)中1-脱氧野尻霉素含量最高(1.545 6 mg/g),黄格鲁(8-15)中1-脱氧野尻霉素含量最低(0.112 5 mg/g)。此方法操作简单,适用于桑叶中1-脱氧野尻霉素提取及含量测定。This study mainly explores the extraction conditions of 1-deoxynojirimycin(DNJ)from mulberry leaves.Determination of 1-deoxynojirimycin(DNJ)content using high-performance liquid chromatography ultraviolet detection method from superior mulberry tree varieties inYuncheng City.Mulberry leaves were ultrasonically extracted with 0.01 mol/L hydrochloric acid,and 1-deoxynojirimycin(DNJ)was derivatized with fluorene methoxycarbonyl chloride(FMOC-Cl)in sodium borate buffer pH 8.5.-Visible detector detection.In this experiment,the ultrasonic extraction conditions were explored,and the extraction conditions were optimized by single factor test and orthogonal test,and the optimal extraction conditions were selected,that is,70℃,350 W ultrasonic extraction for 60 min.the peaks were well separated under these extraction conditions,with no tailing and no continuity.1-Deoxynojirimycin had a good linear relationship in the concentration range of 0.1-1.5 mg/mL(R^(2)=0.9989).The content of 1-deoxynojirimycin in different varieties of mulberry leaves was different.The content of 1-deoxynojirimycin in red big leaves(8-12)was the highest(1.5456 mg/g),and the content of 1-deoxynojirimycin in Huanggelu(8-15)was the highest.1-Deoxynojirimycin had the lowest content(0.1125 mg/g).CONCLUSION:The method is simple to operate and suitable for extraction and content determination of 1-deoxynojirimycin in mulberry leaves.

关 键 词：桑叶 1-脱氧野尻霉素 HPLC 提取条件 

分 类 号：TS207.3[轻工技术与工程—食品科学]