茶皂素对铜绿假单胞菌的抑菌活性及抑菌机制研究  

Study on Bacteriostatic Activity and Mechanism of Inhibition of Pseudomonas aeruginosa by Tea Saponin

作　　者：孙国志 梁宗锁[1,2] 杨东风 张海花[1] Sun Guozhi;Liang Zhongsuo;Yang Dongfeng;Zhang Haihua(College of Life Sciences and Medicine,Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province,Zhejiang Sci-Tech University,Hangzhou 310018;Shaoxing Biomedical Research Institute of Zhejiang Sci-Tech University Co.,Ltd.Zhejiang Engineering Research Center for the Development Technology of Medicinal and Edible Homologous Health Food,Shaoxing 312075)

机构地区：[1]浙江理工大学生命科学与医药学院,浙江省植物次生代谢调控重点实验室,杭州310018 [2]浙江理工大学绍兴生物医药研究院有限公司,药食同源保健食品开发技术浙江省工程研究中心,绍兴312075

出　　处：《中国粮油学报》2024年第8期175-183,共9页Journal of the Chinese Cereals and Oils Association

基　　金：领雁计划项目(2022C02023)。

摘　　要：茶皂素是茶籽中的一种次生代谢产物,主要来自油茶籽榨油后的副产品茶籽饼。为探究其对铜绿假单胞菌抑菌性能以及抑菌机理,扩大油茶副产物茶皂素的开发利用。通过测定茶皂素对铜绿假单胞菌的抑菌圈直径、最小抑菌浓度和最小杀菌浓度来评价茶皂素的抑菌性能,然后通过检测菌体细胞壁细胞膜的完整性以及代谢活动等来探究抑菌机制。实验结果表明,茶皂素对铜绿假单胞菌抑菌圈达到了(23.34±1.52)mm,对铜绿假单胞菌的MIC为15.63 mg/mL,MBC为31.25 mg/mL。抑菌机制实验证实,茶皂素可以破坏细胞膜、细胞壁的完整性,改变其通透性,引起胞内物质(蛋白质、核酸、离子等)的泄漏;降低ATPase和SDH的活性;降低细菌表面的疏水性;茶皂素还可以通过抑制细菌的游动、群集运动、蹭行运动,抑制生物被膜的形成,起到抑制细菌以及减毒效果;并且茶皂素可以与DNA发生相互作用,导致DNA损伤。Tea saponin is a secondary metabolite in tea seeds,mainly from tea seed cake,and it is a by-product of oil extraction from oil tea seeds.In this paper,the bacteriostatic properties against Pseudomonas aeruginosa and the mechanism of bacteriostasis were investigated,and the development and utilization of tea saponin,a by-product of oil tea,was expanded.The bacteriostatic performance of tea saponin was evaluated by determining the diameter of the circle of inhibition,the minimum inhibitory concentration and the minimum bactericidal concentration of Pseudomonas aeruginosa,and then the bacteriostatic mechanism was investigated by detecting the integrity of the cell membrane of the bacterial cell wall as well as the metabolic activity,etc.The experimental results indicated that tea saponin was effective against Pseudomonas aeruginosa.The experimental results indicated that the inhibition circle of tea saponin against Pseudomonas aeruginosa reached(23.34±1.52)mm,the MIC against Pseudomonas aeruginosa was 15.63 mg/mL,and the MBC was 31.25 mg/mL.Bacteriostatic mechanism experiments have confirmed that tea saponin can destroy the integrity of the cell membrane,cell wall,change its permeability,cause the leakage of intracellular substances(proteins,nucleic acids and ions,etc.);reduce the activity of ATPase and SDH;reduce the hydrophobicity of the bacterial surface;tea saponin can also be inhibited by inhibiting the bacterial swimming,swarming,twitching,inhibition of the formation of the biofilm,and play a role in inhibition of the bacteria as well as the effect of toxicity reduction.Tea saponin could interact with DNA and cause DNA damage.

关 键 词：茶皂素 抑菌活性 抑菌机制 铜绿假单胞菌 

分 类 号：TQ455.4[化学工程—农药化工]