地衣酸对胰腺癌细胞系PANC-1的抗增殖作用  

Antiproliferative Effect of Lichenic Acid on Pancreatic Cancer Cell Line PANC-1

作　　者：赵森 周华[1] Zhao Sen;Zhou Hua(School of Basic Medical Science,Anhui Medical College,Hefei 230601,China)

机构地区：[1]安徽医学高等专科学校基础医学院,合肥230601

出　　处：《大理大学学报》2024年第10期26-31,共6页Journal of Dali University

基　　金：安徽省高校自然科学研究重点项目(2023AH052599);安徽省高校哲学社会科学研究重点项目(2023AH052580)。

摘　　要：目的:探讨地衣酸对胰腺癌细胞系PANC-1的抗增殖作用。方法:地衣酸作用于人PANC-1细胞,MTT实验检测细胞增殖活性,流式细胞术检测地衣酸对PANC-1细胞凋亡的影响,划痕实验检测地衣酸对PANC-1细胞迁移的影响,蛋白质印迹法检测BAX、BCL2和TrxR1蛋白的表达,酶标法检测TrxR1的酶活性。结果:地衣酸降低PANC-1细胞活力,促进PANC-1细胞凋亡,显著抑制了PANC-1细胞迁移,上调促凋亡蛋白BAX、下调抗凋亡蛋白BCL2的表达,抑制了TrxR1的表达及酶活性。结论:地衣酸抑制PANC-1增殖,是治疗胰腺癌的潜在化疗药物。Objective:To explore the antiproliferative effect of lichenic acid on pancreatic cancer cell line PANC-1.Methods:Lichenic acid was used to act on human PANC-1 cell,which was further followed by MTT assay to detect cell proliferative activity,flow cytometry to detect the effect of lichenic acid on apoptosis of PANC-1 cell,scratch assay to detect the effect of lichenic acid on the migration of PANC-1 cell,protein blotting to detect the expression of BAX,BCL2,and TrxR1 proteins,and enzyme labeling to detect the enzymatic activity of TrxR1.Results:Lichenic acid reduced PANC-1 cell and promoted the apoptosis of PANC-1 cell,significantly inhibited the migration of PANC-1 cell,up-regulated the expression of pro-apoptotic protein BAX,down-regulated the expression of anti-apoptotic protein BCL2,and also inhibited the expression of TrxR1 and the activity of TrxR1 enzyme.Conclusion:Lichenic acid could inhibit PANC-1 proliferation and is a potential chemotherapeutic agent for pancreatic cancer.

关 键 词：胰腺癌 地衣酸 TrxR 抗肿瘤 

分 类 号：R735.9[医药卫生—肿瘤]