UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1  

作　　者：Shengjie Shi Huan Yuan Lutong Zhang Lei Gao Lili Zhao Xiangfang Zeng Shiyan Qiao Guiyan Chu Chuanjiang Cai 

机构地区：[1]College of Animal Science and Technology,Northwest A&F University,Yangling 712100,Shaanxi,China [2]State Key Laboratory of Animal Nutrition,Ministry of Agriculture Feed Industry Center,China Agricultural University,Beijing 100193,China

出　　处：《Journal of Animal Science and Biotechnology》2024年第5期1894-1907,共14页畜牧与生物技术杂志（英文版）

基　　金：funded by National Key R&D Program of China(NO.2022YFD1300303);National Natural Science Foundation of China(32272849)。

摘　　要：Background The proliferation of porcine ovarian granulosa cells(GCs)is essential to follicular development and the ubiquitin–proteasome system is necessary for maintaining cell cycle homeostasis.Previous studies found that the deubiquitinase ubiquitin carboxyl-terminal hydrolase 1(UCHL1)regulates female reproduction,especially in ovarian development.However,the mechanism by which UCHL1 regulates porcine GC proliferation remains unclear.Results UCHL1 overexpression promoted GC proliferation,and knockdown had the opposite effect.UCHL1 is directly bound to cyclin B1(CCNB1),prolonging the half-life of CCNB1 and inhibiting its degradation,thereby promoting GC proliferation.What's more,a flavonoid compound-isovitexin improved the enzyme activity of UCHL1 and promoted the proliferation of porcine GCs.Conclusions UCHL1 promoted the proliferation of porcine GCs by stabilizing CCNB1,and isovitexin enhanced the enzyme activity of UCHL1.These findings reveal the role of UCHL1 and the potential of isovitexin in regulating proliferation and provide insights into identifying molecular markers and nutrients that affect follicle development.

关 键 词：CCNB1 Granulosa cells Isovitexin PROLIFERATION UCHL1 

分 类 号：S828[农业科学—畜牧学]