The role of singlet oxygen and hydroxyl radical in the photobleaching of meso-substituted cationic pyridyl porphyrins in the presence of folic acid  

作　　者：Lusine Mkrtchyan Torgom Seferyan Marina Parkhats Sergei Lepeshkevich Boris Dzhagarov Gagik Shmavonyan Elena Tuchina Valery Tuchin Grigor Gyulkhandanyan 

机构地区：[1]Laboratory of Bioengineering,Institute of Biochemistry After H.Buniatyan of the NAS of Armenia 5/1 Paruyr Sevak Street,Yerevan 0014,Armenia [2]Laboratory of Biomedical Research,Institute of Biochemistry after H.Buniatyan of the NAS of Armenia 5/1 Paruyr Sevak Street,Yerevan 0014,Armenia [3]B.I.Stepanov Institute of Physics NAS of Belarus 68-2 Nezavisimosti Avenue,Minsk 220072,Belarus [4]National Polytechnic University of Armenia 105 Teryan Street,Yerevan 0009,Armenia [5]Science Medical Centre,Saratov State University 83 Astrakhanskaya Street,Saratov 410012,Russia

出　　处：《Journal of Innovative Optical Health Sciences》2024年第5期67-80,共14页创新光学健康科学杂志（英文）

基　　金：supported by the RA MESCS Science Committee and Belarusian Republican Foundation for Fundamental Research in the frames of the joint research project SC No.21SC-BRFFR-1F007 and BRFFR Grant No.21ARM-014 accordingly,as well as from the Ministry of Science and Higher Education of Russian Federation within the framework of a state assignment(project No.FSRR-2023-0007)。

摘　　要：Using a photosensitizer(PS),light,and oxygen,photodynamic therapy creates cytotoxic reactive oxygen species,such as singlet oxygen(1O2),that kill cancer cells.Many cancer cell lines have up to 300 times more folic acid receptors than healthy cells.Therefore,folic acid is often used to improve selectivity of PSs.Photobleaching poses a disadvantage for PSs.In this paper,we have studied the photoinduced changes of meso-substituted cationic pyridyl porphyrins in the presence of folic acid using uorescence and absorption spectroscopy.In this work,it was demonstrated that L-histidine,which is a 1O2 quencher,and D-mannitol,which is a hydroxyl radical quencher,can reduce photobleaching of cationic porphyrins and their interaction products with FA.This implies both singlet oxygen and hydroxyl radicals are involved in photobleaching.Additionally,our study revealed certain important features of the photobleaching of cationic porphyrins in the presence of folic acid.

关 键 词：Photodynamic therapy cancer quenchers HISTIDINE MANNITOL 

分 类 号：R73[医药卫生—肿瘤]