姜黄素、β-胡萝卜素对太湖猪精液常温保存效果的影响  

Influence of Curcumin andβ-Carotene on the Preservation Effect of Taihu Pig Semen at Room Temperature

作　　者：祁亚东 梁佳玟 侯振业 冯宇辰 赵孝杰 吴怡 吴结革[1] 刘京鸽 QI Ya-dong;LIANG Jia-wen;HOU Zhen-ye;FENG Yu-chen;ZHAO Xiao-jie;WU Yi;WU Jie-ge;LIU Jing-ge(College of Animal Science and Food Engineering,Jinling Institute of Technology,Nanjing,Jiangsu 210000,China)

机构地区：[1]金陵科技学院动物科学与食品工程学院,江苏南京210000

出　　处：《福建农业科技》2024年第7期21-25,共5页Fujian Agricultural Science and Technology

基　　金：国家自然科学基金项目(32002175);金陵科技学院2021年科教融合项目(2021KJRH32);金陵科技学院博士科研启动基金(jit-b-202035);大学生创新创业训练计划(202313573097Y)。

摘　　要：为了探究不同类别的植物源性抗氧化剂对太湖猪精液常温保存效果的影响,选取了姜黄素、β-胡萝卜素两种常见的抗氧化剂,分别以不同浓度(姜黄素:0、3、6、9μg·mL^(−1);β-胡萝卜素:0、10、20、40μg·mL^(−1))添加到太湖猪精液稀释液中,采用目测法、低渗肿胀法和瑞氏-吉姆萨染色法检测精子在17℃保存环境中第1、3、5、7 d的活力、质膜完整率和顶体完整率。结果表明:添加一定浓度的姜黄素、β-胡萝卜素均能显著提高精子质量,其中姜黄素的最适添加浓度为6μg·mL^(−1),β-胡萝卜素的最适添加浓度为20μg·mL^(−1)。In order to explore the effect of different types of phytogenic antioxidants on the preservation of Taihu pig semen at room temperature,the two commonly used antioxidants,curcumin andβ-carotene,were selected.They were added at different concentrations(curcumin:0,3,6,9μg·mL^(−1);β-carotene:0,10,20,40μg·mL^(−1))to the semen diluent of Taihu pig.The sperm vitality,plasma membrane integrity rate and acrosome integrity rate were detected by using the visual observation,hypotonic swelling method and Wright-Giemsa staining method on the 1 st,3 rd,5 th and 7 th day of preservation at 17°C.The results showed that the addition of certain concentrations of curcumin andβ-carotene could significantly improve the sperm quality,with the optimal concentrations being 6μg·mL^(−1)for curcumin and 20μg·mL^(−1)forβ-carotene.

关 键 词：猪精液 常温保存 姜黄素 Β-胡萝卜素 

分 类 号：S828[农业科学—畜牧学]