近交系自发性2型糖尿病中国地鼠山医群体血液生理生化指标的检测分析  

Analysis of blood physiological and biochemical indicators of spontaneous type 2 diabetes in inbred SHANXI MU Chinese hamsters

作　　者：杨钰婷 杨依纯 卫兵艳 武剑琴 张瑞虎 陈朝阳 YANG Yuting;YANG Yichun;WEI Bingyan;WU Jianqin;ZHANG Ruihu;CHEN Zhaoyang(Laboratory Animal Center of Shanxi Medical University,Taiyuan 030001,China;Shanxi Key Laboratory of Experimental Animal Science and Animal Model of Human Diseases,Taiyuan 030001)

机构地区：[1]山西医科大学实验动物中心,太原030001 [2]山西省实验动物与人类疾病动物模型重点实验室,太原030001

出　　处：《中国比较医学杂志》2024年第9期19-23,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(U21A20194);山西省科技厅,中央引导地方科技发展资金项目(YDZJSX2022B009)。

摘　　要：目的对课题组前期建立的近交系自发性2型糖尿病(T2DM)中国地鼠山医群体的血液生理生化指标进行检测并分析。方法选取12月龄近交系自发性T2DM中国地鼠山医群体及正常地鼠各10只,进行空腹采血,应用Sysmex XT自动血液分析仪及Hitachi全自动生化仪对血液15项生理指标及16项生化指标进行测定并进行统计分析。结果与对照组相比,糖尿病组白细胞(WBC)、红细胞(RBC)、血小板(PLT)、血红蛋白(HGB)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、血糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、尿酸(UA)等指标差异具有统计学意义(P<0.05)。结论对课题组前期建立的近交系中国地鼠自发性T2DM模型进行血液生理生化指标检测分析,其变化符合人类T2DM发病的趋势,为该模型的应用及发展提供了基础数据。Objective To determine the blood physiological and biochemical indexes in the inbred SHANXI MU strain of spontaneous type 2 diabetes(T2DM)Chinese hamsters.Methods Chinese hamsters with spontaneously developed T2DM and normal hamsters(n=10 hamsters per group),aged 12 months,were selected for the study.Fasting blood samples were collected and 15 physiological parameters and 16 biochemical indicators were analyzed using a Sysmex XT automated hematology analyzer and Hitachi fully automatic biochemical analyzer.Results The white cell count,red cell count,platelet count,hemoglobin level,alanine transaminase,aspartate transaminase,glutamate,total cholesterol,triglycerides,and uric acid all differed significantly between the diabetic and control groups(P<0.05).Conclusions The change of blood physiological and biochemical indexes in the Chinese hamster spontaneous T2DM model were in line with the trend in human T2DM incidence,thus providing basic data for the application of this model.

关 键 词：中国地鼠 2型糖尿病 生理指标 生化指标 

分 类 号：R-33[医药卫生]