聚肌胞苷酸复合游泳诱导的慢性疲劳综合征模型小鼠行为学中品系及性别差异研究  

Strain and gender differences in behavioral models of chronic fatigue syndrome induced by polyinosinic-polycytidylic acid and swimming in mice

作　　者：田骄 苏芮 王婷婷 裴海鸾 王晶[1] TIAN Jiao;SU Rui;WANG Tingting;PEI Hailuan;WANG Jing(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Beijing Tongrentang Technologies Co.Ltd Pharmaceutical Factory,Beijing 100071)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]北京同仁堂科技发展股份有限公司制药厂,北京100071

出　　处：《中国比较医学杂志》2024年第9期24-33,共10页Chinese Journal of Comparative Medicine

基　　金：国家重点研发计划(2019YFC1711400)。

摘　　要：目的比较ICR和C57BL/6J两个品系小鼠建立的慢性疲劳综合征模型行为学中品系及性别的差异,为慢性疲劳综合征模型动物的选择提供实验依据。方法ICR和C57BL/6J小鼠,雌雄各半,通过每隔3 d腹腔注射聚肌胞苷酸(polyinosinic-polycytidylic acid,Poly I:C)复合每天游泳,连续造模15 d,造模期间测定小鼠体重、进食量并进行疲劳量表评分,造模结束后进行行为学测定,包括力竭游泳、悬尾、机械痛阈和高架十字迷宫。结果与对照组相比,两种品系模型组小鼠力竭游泳时间均显著缩短(P<0.05、P<0.01),悬尾不动时间显著延长(P<0.05、P<0.01),机械痛阈值显著降低(P<0.05、P<0.01),两品系雄性模型鼠高架十字迷宫进入开放臂时间及次数显著降低(P<0.05、P<0.01),C57BL/6J雄性模型鼠体重显著降低(P<0.05、P<0.01),ICR雌性模型鼠体重显著降低(P<0.05)后有所升高。C57BL/6J对照组小鼠力竭游泳时间显著低于ICR对照组小鼠(P<0.01)。C57BL/6J模型组小鼠悬尾不动时间显著高于ICR模型组小鼠(P<0.01)。结论两品系小鼠在体重、疲劳程度及抑郁程度方面均有差异;同品系小鼠的雌性与雄性之间存在差异,雄性鼠焦虑程度重于雌性鼠。Objective To compare strain and gender differences between ICR and C57BL/6J mouse chronic fatigue syndrome models and provide experimental evidence for the selection of model animals for chronic fatigue syndrome.Methods ICR and C57BL/6J mice(half male and half female)were injected intraperitoneally with polyinosinic-polycytidylic acid(Poly I:C)every three days and forced to swim daily.The modeling was performed for 15 consecutive days,during which the weight and food intake of the mice were measured,and fatigue scores were assessed.After the modeling was completed,behavioral tests were carried out,including exhaustive swimming,tail suspension,mechanical pain threshold,and elevated plus maze.Results Compared with the control group,both strains of model mice had significantly decreased exhaustion times in the exhaustive swimming test(P<0.05,P<0.01),significantly increased immobility time in the tail suspension test(P<0.05,P<0.01),and a significantly decreased mechanical pain threshold(P<0.05,P<0.01),and male model mice had significantly decreased open arm entry time and frequency in the elevated plus maze(P<0.05,P<0.01).The weight of model male C57BL/6J mice significantly decreased(P<0.05,P<0.01).The weight of model female ICR mice increased after a significant reduction(P<0.05).The exhaustion time of control C57BL/6J mice was significantly lower than that of control ICR mice(P<0.01).The immobility time of model C57BL/6J mice was significantly greater than that of model ICR mice(P<0.01).Conclusions There were differences between the two strains of mice in terms of weight change,fatigue level,and depression.Within the same strain,there were differences between males and females,and the anxiety level of males was higher than that of females.

关 键 词：慢性疲劳综合征 聚肌胞苷酸 病毒感染 品系差异 

分 类 号：R-33[医药卫生]