异绿原酸对肉鸡生长性能、养分表观代谢率及脂多糖应激状态下抗氧化能力的影响  

Effects of Isochlorogenic Acid on Growth Performance,Nutrient Apparent Metabolic Rates of Broilers and Antioxidant Capacity of Broilers under Lipopolysaccharide Stress

作　　者：江皓天 周思源 王雨佳 赵紫茵 韦旭 颜安 刘国华[2] 刘树栋[1] 陈宝江[1] JIANG Haotian;ZHOU Siyuan;WANG Yujia;ZHAO Ziyin;WEI Xu;YAN An;LIU Guohua;LIU Shudong;CHEN Baojiang(College of Animal Science and Technology,Hebei Agricultural University,Baoding 071001,China;Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100080,China)

机构地区：[1]河北农业大学动物科技学院,保定071001 [2]中国农业科学院饲料研究所,北京100080

出　　处：《动物营养学报》2024年第9期5630-5641,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：河北省自然科学基金资助项目(C2024204217,C2023204288);农业农村部饲料生物技术重点实验室开放课题;河北省农业产业技术体系创新团队(HBCT2023210407)。

摘　　要：本试验旨在研究异绿原酸(ICGA)对肉鸡生长性能、养分表观代谢率以及脂多糖(LPS)刺激肉鸡抗氧化能力的影响。选取320只体重相近的1日龄健康科宝肉雏鸡,按2×2双因素试验设计(有或无ICGA干预;有或无LPS处理)随机分为4组(CON组、L-CON组、ICGA组和L-ICGA组),每组8个重复,每个重复10只鸡。CON组和L-CON组饲喂基础饲粮,ICGA组和L-ICGA组在基础饲粮中添加2000 mg/kg的ICGA。L-CON组和L-ICGA组肉鸡于42日龄按照200μg/kg BW的剂量胸肌注射LPS,CON组和ICGA组注射等量生理盐水,注射2 h后取样测定抗氧化指标。结果显示:1)饲粮中添加ICGA显著增加了肉鸡的平均日增重和末重(P<0.05),显著降低了料重比(P<0.05);2)饲粮中添加ICGA显著提高了肉鸡对粗蛋白质和钙的表观代谢率(P<0.05);3)饲粮中添加ICGA可以逆转因LPS刺激导致的肉鸡血清谷丙转氨酶和碱性磷酸酶活性的增加(P<0.05),同时使血清高密度脂蛋白胆固醇含量显著提升(P<0.05);4)饲粮中添加ICGA可以显著上调LPS刺激肉鸡回肠超氧化物歧化酶-1、谷胱甘肽过氧化氢酶的mRNA相对表达量(P<0.05),显著增加血清、回肠及肝脏中超氧化物歧化酶活性以及血清和回肠中谷胱甘肽过氧化氢酶活性(P<0.05),并显著降低血清和回肠中丙二醛含量(P<0.05)。综上所述,ICGA可改善肉鸡的生长性能及粗蛋白质和钙的表观代谢率,并且可以通过调控血清脂质代谢、上调抗氧化基因表达、提高抗氧化酶活性以及降低脂质过氧化物产物等途径应对LPS刺激引起的氧化应激。This experiment was conducted to study the effects of isochlorogenic acid(ICGA)on the growth performance,nutrient apparent metabolic rates of broilers,as well as the antioxidant capacity of broilers challenged by lipopolysaccharide(LPS).A total of 320 one-day-old healthy Cobb broilers with similar body weight were selected according to a 2×2 two-factor experimental design(with or without ICGA intervention;with or without LPS-induced)were randomly divided into 4 groups:CON group,L-CON group,ICGA group and L-ICGA group.Each group had 8 replicates,with 10 broilers in each replicate.Broilers in the CON group and L-CON group were fed a basal diet,while those in the ICGA group and L-ICGA group were fed the basal diet supplemented with 2000 mg/kg of ICGA.At 42 days of age,broilers in L-CON group and L-ICGA group were injected with LPS at 200μg/kg BW in breast muscle,and broilers in the CON group and ICGA group were injected with the same amount of normal saline.The antioxidant indexes were measured by sampling 2 hours after the injection.The results showed as follows:1)diet supplemented with ICGA significantly increased the average daily gain and final body weight of broilers(P<0.05),and significantly reduced the feed to gain ratio of broilers(P<0.05);2)diet supplemented with ICGA significantly increased the apparent metabolic rates of crude protein and calcium of broilers(P<0.05);3)diet supplemented with ICGA could reverse the increase of serum alanine aminotransferase and alkaline phosphatase activity caused by LPS challenge(P<0.05),at the same time,the content of serum high density lipoprotein cholesterol was significantly increased(P<0.05);4)diet supplemented with ICGA could significantly up-regulate the mRNA relative expression levels of superoxide dismutase-1 and glutathione catalase in ileum(P<0.05),significantly increase the superoxide dismutase activity in serum,ileum and liver and the activity of glutathione catalase in serum and ileum(P<0.05),and significantly decrease the content of malondialdehyde in seru

关 键 词：异绿原酸 肉鸡 脂多糖 生长性能 表观代谢率 抗氧化能力 

分 类 号：S816[农业科学—饲料科学]