植物乳杆菌RG-034耐酸菌粉在大鼠结肠内容物中分布的研究  

Distribution of Lactobacillus Plantarum RG-034 Acid-resistant Powders in the Colon Contents of Rats

作　　者：张沂 许锐鹏 徐冯莲 李煜龙 陈贵浩 方伟蓉 ZHANG Yi;XU Ruipeng;XU Fenglian;LI Yulong;CHEN Guihao;FANG Weirong(School of Basic Medicine and Clinical Pharmacy,China Pharmaceutical University,Nanjing 211198,China;Guangdong Qiangji Pharmaceutical Co.,Ltd.,Chaozhou,Guangdong 515638,China)

机构地区：[1]中国药科大学基础医学与临床药学学院,南京211198 [2]广东强基药业有限公司,广东潮州515636

出　　处：《药学与临床研究》2024年第4期304-307,共4页Pharmaceutical and Clinical Research

摘　　要：目的:研究耐酸包埋技术对植物乳杆菌(L.plantarum)RG-034在结肠中分布的作用。方法:通过菌株培养、发酵、离心和耐酸保护剂包埋制备L.plantarum RG-034耐酸菌粉,并测定耐酸后活菌数。雄性SD大鼠分别灌胃L.plantarum RG-034菌粉(1.0×10^(11)CFU)、L.plantarum RG-034耐酸菌粉(3.05×10^(10)CFU),灌胃后6、12、24 h,检测大鼠小肠、结肠L.plantarum RG-034浓度。结果:制备的L.plantarum RG-034耐酸菌粉浓度为6.1×10^(10)CFU·g^(-1),体外耐酸后L.plantarum RG-034活菌数约为1.4×10^(10)CFU·g^(-1)。与空白对照组相比,菌粉组及耐酸菌粉组大鼠小肠和结肠肠段内容物中L.plantarum RG-034的浓度均有所升高。在结肠的分布中,菌粉给药6 h后已进入结肠,随后排出体外,给药后24 h浓度逐渐降低,而耐酸菌粉给药6 h后小肠中浓度高于结肠,12 h后结肠中浓度明显上升,24 h后结肠中浓度较高。结论:与L.plantarum RG-034菌粉组相比,L plantarum RG-034耐酸菌粉组L.plantarum结肠浓度较高,提高了L.plantarum在肠道中的活力。Objective:To study the effects of acid-resistant embedding technique on colon distribution of Lactobacillus plantarum(L.plantarum)RG-034.Methods:L.plantarum RG-034 acid-resistant powders were prepared by strain culture,fermentation,centrifugation and embedding with acid-resistant protectants.The number of viable bacteria after acid-resistance was determined.Every male Sprague Dawley(SD)rat was i.g.administrated with L.plantarum RG-034 powders(1.0×10^(11)CFU)or L.plantarum RG-034 acid-resistant powders(3.05×10^(10)CFU),and the concentrations of L.plantarum RG-034 were measured in the small intestines and the colons by real time PCR at 6,12,and 24 h after administration.Results:The results showed that the concentration of L.plantarum RG-034 was 6.1×10^(10)CFU·g^(-1)in the acid-resistant powders,and the number of live bacteria of L.plantarum RG-034 after acid-tolerance was about 1.4×10^(10)CFU·g^(-1).The concentrations of L.plantarum RG-034 increased in the contents of intestinal segments of both the small intestines and the colons in the groups of L.plantarum RG-034 powders compared with those in the control group.The concentrations of L.plantarum RG-034 in the contents of small and colon intestinal segments of rats in the L.plantarum RG-034 acid-resistant powder group were significantly increased.It was further found that L.plantarum RG-034 from the L.plantarum RG-034 powders entered colon after 6 h of administration and subsequently excreted from the body,and the concentrations gradually decreased 24 h after administration.The concentrations of L.plantarum RG-034 for the L.plantarum RG-034 acid-resistant powder group in small intestines were higher than those in colons after 6 h of administration,the concentrations in colon increased significantly after 12 h and were higher after 24 h.Conclusion:Compared with the L.plantarum RG-034 powders,the L.plantarum RG-034 acid-resistant powders reaches colons at higher concentrations,which improves their viability in intestines and provides a basis for clinical appli

关 键 词：植物乳杆菌RG-034 耐酸菌粉 益生菌 

分 类 号：TS201.3[轻工技术与工程—食品科学] R96[轻工技术与工程—食品科学与工程]