急慢性牙髓炎患者血清PGE2、BMP-2表达及其对病情转归的影响  

The difference of serum PGE2 and BMP⁃2 expression in patients with acute and chronic pulpitis and their relationship with the outcome of the disease

作　　者：邓锐 田瑞雪 张玉 程晨 王宇 DENG Rui;TIAN Ruixue;ZHANG Yu;CHENG Chen;WANG Yu(Department of Stomatology,Shouxian People's Hospital,Shouxian,Anhui,China,232200;Department of Stomatology,the First Affiliated Hospital of Bengbu Medical University,Bengbu,Anhui,China,233000)

机构地区：[1]安徽寿县人民医院口腔科,安徽寿县232200 [2]安徽蚌埠医科大学第一附属医院口腔科,安徽蚌埠233000

出　　处：《分子诊断与治疗杂志》2024年第9期1797-1800,1805,共5页Journal of Molecular Diagnostics and Therapy

基　　金：安徽省高校自然科学研究项目(KJ2021A0790)。

摘　　要：目的分析急慢性牙髓炎患者血清前列腺素E2(PGE2)、骨形态发生蛋白-2(BMP-2)表达及其对病情转归的影响。方法选择2020年6月至2023年5月安徽寿县人民医院收治的110例牙髓炎患者作为研究对象,其中急性牙髓炎50例(急性组),慢性牙髓炎60例(慢性组),根据疾病治疗转归情况分为预后不良组(n=26)、预后良好组(n=84)。另选取同期体检健康者76名作为对照组。采用酶联免疫吸附法检测所有研究对象血清PGE2、BMP-2表达水平。采用受试者工作特征曲线(ROC)评估血清PGE2、BMP-2对急慢性牙髓炎患者疾病转归的诊断价值,采用多因素Logistic回归分析影响急慢性牙髓炎患者疾病转归的因素。结果血清PGE2水平:急性牙髓炎组>慢性牙髓炎组>对照组,BMP-2水平:急性牙髓炎组<慢性牙髓炎组<对照组,差异有统计学意义(P<0.05)。预后不良组血清CRP、TNF-α、PGE2水平均高于预后良好组,血清BMP-2低于预后良好组,差异有统计学意义(P<0.05)。ROC曲线分析显示,血清PGE2、BMP-2及二者联合评估急慢性牙髓炎患者疾病转归的AUC分别为0.833(95%CI:0.783~0.883)、0.866(95%CI:0.816~0.916)、0.914(95%CI:0.864~0.964)。多因素分析显示,血清CRP>644.42 mg/L(OR=2.675,95%CI:1.701~4.207)、TNF-α>57.73 ng/mL(OR=2.779,95%CI:1.689~4.571)、PGE2>133.45 pg/m(OR=4.154,95%CI:2.116~8.152)、BMP-2≤89.73 g/L(OR=3.501,95%CI:1.987~6.168)均为影响急慢性牙髓炎患者预后不良的危险因素(P<0.05)。结论急慢性牙髓炎患者血清PGE2水平升高,BMP-2水平降低,二指标水平与疾病转归密切相关,可作为评估急慢性牙髓炎患者疾病转归的生物学标志物。Objective To analyze the expression difference of serum prostaglandin E2(PGE2)and bone morphogenetic protein-2(BMP-2)in cases with acute and chronic pulpitis and their relationship with the outcome of the disease.Methods A total of 110 cases of pulpitis from June 2020 to May 2023 were se-lected,including 50 cases of acute pulpitis(acute group)and 60 cases of chronic pulpitis(chronic group).These cases were then divided into a poor prognosis group(n=26)and a good prognosis group(n=84)based on treatment outcomes.Another 76 healthy subjects were selected as a control group.The expression levels of serum PGE2 and BMP-2 were detected using ELISA.The diagnostic value of serum PGE2 and BMP-2 in pre-dicting disease outcomes for patients with acute and chronic pulpitis was evaluated using ROC analysis.Fur-thermore,factors influencing the disease outcomes of patients with acute and chronic pulpitis were explored through multivariate logistic regression.Results The level of serum PGE2 in the acute pulpitis group was higher than that in the chronic pulpitis group and the control group(acute pulpitis group>chronic pulpitis group>control group).The level of BMP-2 was lower than that in the chronic pulpitis group and the control group(acute pulpitis group<chronic pulpitis group<control group),and the difference was statistically significant(P<0.05).The levels of serum CRP,TNF-α,and PGE2 in the poor prognosis group were higher than those in the good prognosis group.Serum BMP-2 was lower than those in the good prognosis group,and the difference was statistically significant(P<0.05).ROC curve analysis showed that the disease outcome AUC of serum PGE2,BMP-2 and their combination in patients with acute and chronic pulpitis were 0.833(95%CI:0.783~0.883),0.866(95%CI:0.816~0.916)and 0.914(95%CI:0.864~0.964),respectively.Multivariate analysis showed that serum CRP>644.42 mg/L(OR=2.675,95%CI:1.701~4.207),TNF-α>57.73 ng/mL(OR=2.779,95%CI:1.689-4.571),PGE2>133.45 pg/m(OR=4.154,95%CI:2.116~8.152)and BMP-2≤89.73 g/L(OR=3.501,95%CI:1.987-

关 键 词：急慢性牙髓炎 前列腺素E2 骨形态发生蛋白-2 疾病转归 

分 类 号：R781.31[医药卫生—口腔医学]