基于Mirau干涉结构的全场光学相干层析系统  

Full⁃Field Optical Coherence Tomography System Based on Mirau Interference Structure

作　　者：何豪 李俊[1] 邹劲松[1] 尹韶云[1] 王金玉 He Hao;Li Jun;Zou Jinsong;Yin Shaoyun;Wang Jinyu(Chongqing Institute of Green and Intelligent Technology,Chinese Academy of Sciences,Chongqing 400714,China;Chongqing School,University of Chinese Academy of Sciences,Chongqing 400714,China)

机构地区：[1]中国科学院重庆绿色智能技术研究院,重庆400714 [2]中国科学院大学重庆学院,重庆400714

出　　处：《中国激光》2024年第15期38-47,共10页Chinese Journal of Lasers

基　　金：中国科学院仪器研制项目(E028610101);重庆市科技局重大仪器专项(Y92A140Y10)。

摘　　要：基于Linnik结构的全场光学相干层析系统以高分辨率、深穿透性、无损非接触、数百万通道并行层析等优点被广泛应用于生物医学成像领域。在该系统中,参考臂和采样臂采用两个完全相同的显微物镜,二者的对准精度均为微米级,无法通过机械结构的约束使其满足成像条件。在不同的应用场景下成像时,需要同时更换两个物镜并需要重新调校,过程比较繁琐。在环境振动以及温湿度和结构应力变化的工况下,系统性能会降低甚至失效。针对上述问题,本课题组提出了基于Mirau干涉结构的全场光学相干层析系统,将参考臂和采样臂集成到一个方向上,同时,针对样品的折射率特性,在物镜结构中填充相应的介质进行折射率匹配。该系统性能稳定,并且可以随意切换倍率。采用该系统对非生物样本(PCB板)和生物样本(洋葱表皮和绿萝叶片)进行了成像实验,验证了该系统的可行性。Objective Full-field optical coherence tomography(FFOCT)is a contactless,high-resolution,real-time imaging method based on OCT technology.It utilizes the backscattering ability of the internal structure of the tissue to obtain a light signal with structural information about the tissue through the detector and then restores the real internal structure through a computer.With the introduction of the Linnik interferometer structure with two identical large numerical aperture microscope objectives into the FFOCT system,the imaging resolution of the system has been improved to the submicron level.Therefore,scholars have begun to conduct further research on aspects such as system effectiveness,stability,practicality,cost,and efficiency.Because the reference and sample arms use two identical microscope objectives,their alignment accuracy is at the micron level,which cannot meet the imaging conditions by the constraints of the mechanical structure.The presence of these two microscope objectives also increases the cost of the system.For different application scenarios,both objectives should be replaced and recalibrated simultaneously,which is a cumbersome process.In this study,we built an FFOCT system based on a Mirau interference structure,integrated the reference arm in the original structure into a self-made Mirau attachment,and assembled it between the objective lens of the sample arm and the sample.This makes the system more compact and stable and reduces costs.The microscope objective at different magnifications can be replaced according to requirements,and different media can be used for index matching.Methods Based on the imaging principle of the FFOCT system,we first simulated the Mirau interference structure using the optical simulation software VirtualLab Fusion.In the preliminary verification stage,only the Mirau-attachment structure was designed;thus,the microscope objective was simplified to an ideal lens that satisfied the software parameters.The feasibility of the solution was verified through light-field

关 键 词：全场光学相干层析 显微物镜 环境振动 Mirau干涉结构 折射率匹配 

分 类 号：TH744[机械工程—光学工程]