克氏原螯虾3群体双列杂交群体耐高温性能比较  

Comparison of high temperature tolerance in three population diallel hybrid population of red swamp crayfish(Procambarus clarkii)

作　　者：吴玮杰 徐啟洲 万通 贾亮[1] 程熙 李典中 白志毅[1,4] WU Weijie;XU Qizhou;WAN Tong;JIA Liang;CHENG Xi;LI Dianzhong;BAI Zhiyi(Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries,Shanghai Ocean University,Shanghai 201306,China;Shanghai Jiading District Fisheries Technology Promotion Station,Shanghai 201800,China;Wuhu Shengdian Leisure Ecological Park Company,Wuhu 241212,China;Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学农业农村部淡水水产种质资源重点实验室,上海201306 [2]上海市嘉定区水产技术推广站,上海201800 [3]芜湖盛典休闲生态园有限公司,安徽芜湖241212 [4]上海海洋大学上海市水产动物良种创制与绿色养殖协同创新中心,上海201306

出　　处：《大连海洋大学学报》2024年第4期606-612,共7页Journal of Dalian Ocean University

基　　金：上海市科技创新行动计划项目(23N11900300);安徽省水产种业振兴项目(2022AHNYNC081FW)。

摘　　要：为构建耐高温性状的克氏原螯虾(Procambarus clarkii)杂交群体,以芜湖、建湖、滆湖3个克氏原螯虾群体完全双列杂交的9个F_(1)群体为亲本,通过自交获得F_(2)群体[滆湖♀×建湖♂(GJ)、建湖♀×滆湖♂(JG)、滆湖♀×芜湖♂(GW)、芜湖♀×滆湖♂(WG)、建湖♀×芜湖♂(JW)、芜湖♀×建湖♂(WJ)、滆湖♀×滆湖♂(GG)、建湖♀×建湖♂(JJ)、芜湖♀×芜湖♂(WW)],采用耐热性(upper thermal tolerance,UTT)、心率的阿伦尼乌斯拐点温度(arrhenius break temperatures,ABT)作为评定指标,并结合临界温度法(critical thermal methodology,CTM)、半致死时间(half leathaltime,LT_(50))等方法,比较了9个F_(2)群体对高温的耐受性,并进一步分析了克氏原螯虾UTT值、ABT值与体质量等性状的相关性。结果表明:各群体UTT值排序为GW>GJ>WJ>JG>GG>WW>WG>JW>JJ,且GW群体UTT值显著高于其余群体(P<0.05);各群体ABT排序为GW>WJ>WW>WG>JW>JJ>JG>GJ>GG,且GW群体ABT显著高于其余群体(P<0.05);各群体CTMax排序为GW>WJ>GJ>GG>WW>JG>JW>WG>JJ,且GW、WJ、GJ群体显著高于其余群体(P<0.05);各群体LT_(50)排序为GW>GJ>WJ>JG>WW>GG>JW=JJ>WG;不同规格克氏原螯虾个体之间耐热性(UTT)存在显著性差异(P<0.05),即克氏原螯虾虾苗耐热性显著高于初达上市规格的商品虾(P<0.05);克氏原螯虾热胁迫前后心率均与体质量呈负相关,相关系数分别为-0.21和-0.15(P<0.05)。研究表明,滆湖♀×芜湖♂(GW)群体4个耐高温指标均优于其他群体,与UTT等指标相比,采用ABT指标测定对个体损害小,更适用于良种选育。In order to establish a hybrid population of red swamp crayfish(Procambarus clarkii)with high temperature tolerance,nine F_(1)populations of complete diallel hybridization from three populations of red swamp crayfish,Wuhu,Jianhu,and Gehu,were used as broodstocks to obtain F_(2)populations through self pollination[Gehu♀×Jianhu♂(GJ),Jianhu♀×Gehu♂(JG),Gehu♀×Wuhu♂(GW),Wuhu♀×Gehu♂(WG),Jianhu♀×Wuhu♂(JW),Wuhu♀×Jianhu♂(WJ),Gehu♀×Gehu♂(GG),Jianhu♀×Jianhu♂(JJ),and Wuhu♀×Wuhu♂(WW)]exposed to the water temperature from 30℃to 35℃at raising rate of 1℃/2 h,and at raising rate of 0.5℃/4 h until the crayfish death.The tolerance of 9 F_(2)populations to high temperature was compared using evaluation indicators of upper thermal tolerance(UTT)and Arrhenius Break Temperatures(ABT)of heart rate,combined with Critical Thermal Methodology(CTM),median lethal Time(LT_(50))and other methods,and the correlation was analyzed between ABT values and traits including body weight.The results showed that the descending order of the UTT values in each group were ranked as GW>GJ>WJ>JG>GG>WW>WG>JW>JJ,with significantly higher in the GW group than that in the other groups(P<0.05).The ABT was expressed as GW>WJ>WW>WG>JW>JJ>JG>GJ>GG in each group,significantly higher in the GW group than that in the other groups(P<0.05).The CTMax was found to be GW>WJ>GJ>GG>WW>JG>JW>WG>JJ in different groups,significantly higher in the GW,WJ,and GJ groups than that in the other groups(P<0.05).The LT_(50)was described as GW>GJ>WJ>JG>WW>GG>JW=JJ>WG in the red swamp crayfish in each group.There was significant difference in heat tolerance(UTT)among individuals in the crayfish with different developmental stages(P<0.05),significantly higher heat tolerance in crayfish juveniles than that in remarkable crayfish just reached the market specifications(P<0.05).Before and after heat stress,there was a negative correlation between heart rate and body weight in crayfish,with correlation coefficients of-0.21 and-0.15,respectively

关 键 词：克氏原螯虾 耐高温性能 耐热性 心率拐点温度 群体 

分 类 号：S966.12[农业科学—水产养殖]