酿酒酵母转座子介导外源基因的整合效率  

The integration efficiency of heterologous gene mediatedby transposons in Saccharomyces cerevisiae

作　　者：王丹丹 南辰辰 李宪臻[1] 杨帆[1] WANG Dandan;NAN Chenchen;LI Xianzhen;YANG Fan(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2024年第5期349-354,共6页Journal of Dalian Polytechnic University

基　　金：辽宁省自然科学基金项目(2020-MS-276);辽宁省教育厅科学技术研究项目(J2020041).

摘　　要：为了比较分析酿酒酵母不同转座子对外源基因整合效率的影响,构建了转座子Ty1、Ty2和Ty4介导的G418表达盒及酿酒酵母BY4741重组菌BY-Ty1、BY-Ty2、BY-Ty4。研究发现,Ty2介导的整合型表达G418的BY4741重组菌转化平均菌落数为122,整合效率为93.33%,均高于Ty1和Ty4。Ty2介导的G418抗性重组菌BY-Ty2,比BY-Ty1和BY-Ty4对G418抗耐受性更强,基因拷贝数更高。结果表明,转座子Ty2更适合在酿酒酵母中介导外源基因整合。To compare and analyze the effects of different transposons on the integration efficiency of exogenous genes in Saccharomyces cerevisiae , G418 expression cassettes were constructed, mediated by transposons Ty1, Ty2, and Ty4, as well as recombinant strains BY-Ty1, BY-Ty2 and BY-Ty4 of BY4741. It was found that the average colony count of BY4741 recombinant strains expressing G418 mediated by Ty2 was 122 and the integration efficiency was 93.33%, both of which were higher than those of Ty1 and Ty4. G418 resistant recombinant strain BY-Ty2 mediated by Ty2 has stronger resistance to G418 than BY-Ty1 and BY-Ty4, with higher gene copy numbers. The results indicated that transposon Ty2 was more suitable for mediating exogenous gene integration in Saccharomyces cerevisiae .

关 键 词：酿酒酵母 转座子 基因整合 G418 

分 类 号：Q812[生物学—生物工程]