基于斑马鱼失眠症模型评价牦牛乳全脂乳粉改善睡眠功效  

Assessment of the Sleep-Improving Efficacy of Yak Milk Powder Based on a Zebrafish Insomnia Model

作　　者：李一澍 崔广智 苏德亮 宋礼 LI Yishu;CUI Guangzhi;SU Deliang;SONG Li(Gansu Hualing Dairy Co.,Ltd.,Gannan 747000;Gannan Yak Milk Research Institute,Gannan 747000)

机构地区：[1]甘肃华羚乳品股份有限公司,甘南747000 [2]甘南牦牛乳研究院,甘南747000

出　　处：《食品工业》2024年第8期78-82,共5页The Food Industry

摘　　要：基于斑马鱼失眠症模型评价牦牛乳全脂乳粉对睡眠的影响。将斑马鱼随机分成6组,分为正常对照组(水)、模型对照组(戊四唑, Pentetrazol, PTZ)、褪黑素组和不同质量浓度的牦牛乳全脂乳粉组(低剂量组、中剂量组和高剂量组);采用最大耐受度(maximum tolerated dose, MTC)筛选出牦牛乳全脂乳粉的最大的功效浓度;采用动物学行为分析法测定斑马鱼的觉醒活动量和觉醒总时间;采用q-PCR检测mtnr1aa和gabra1基因相对表达量。结果提示,牦牛乳全脂乳粉的最大功效浓度为2 000μg/mL;在其干预下,斑马鱼的觉醒活动量和觉醒总时间显著减弱,并且显著上调mtnr1aa和gabra1基因相对表达量。牦牛乳全脂乳粉具有减弱PTZ诱导的斑马鱼失眠症的作用,但其具体作用机理仍需进行深入研究。Evaluate the effect of whole yak milk powder on sleep in a zebrafish insomnia model.Zebrafish were randomly divided into 6 groups,including normal control group,model control group(pentylenetetrazol,PTZ),melatonin group,and different quality concentration groups of whole yak milk powder(low-dose group,medium-dose group,and high-dose group).The maximum tolerated concentration(MTC)was used to screen for the maximum effective concentration of whole yak milk powder.Zebrafish arousal activity and total arousal time were determined using ethological behavior analysis.The relative expression levels of mtnr1aa and gabra1 genes were detected using q-PCR.The results suggested that the maximum effective concentration of whole yak milk powder was 2000μg/mL.Under its intervention,the arousal activity and total arousal time of zebrafish were significantly reduced,and the relative expression levels of mtnr1aa and gabra1 genes were significantly upregulated.Whole yak milk powder has a sleep-promoting effect on PTZ-induced zebrafish insomnia,but its specific mechanism of action requires further investigation.

关 键 词：牦牛乳全脂乳粉 斑马鱼 失眠症 

分 类 号：TS252.51[轻工技术与工程—农产品加工及贮藏工程] TS201.4[轻工技术与工程—食品科学与工程]