饲料中异硫氰酸酯的测定与分析  

Determination and Analysis of Isothiocyanate in Feed

作　　者：曹琥靓 施嵘 CAO Huliang;SHI Rong(Shanghai Yuanben Food Quality Inspection Co.,Ltd.,Shanghai 200090)

机构地区：[1]上海源本食品质量检验有限公司,上海200090

出　　处：《食品工业》2024年第8期266-269,共4页The Food Industry

摘　　要：饲料中的异硫氰酸酯(Isothiocyanates)的含量直接影响动物的生长和健康,因此快速准确地测定饲料中异硫氰酸酯含量成为了农业生产中的一项重要任务。文章旨在建立以丁基异硫氰酸酯为内标测定饲料中异硫氰酸酯含量的方法,为农业生产和检验检测提供科学依据和技术支持。在提取饲料中的异硫氰酸酯在试验中,选取了猫粮作为样品,提取饲料中的异硫氰酸酯,并使用气相色谱-质谱法(GC-MS)对其进行了分析。同时,利用加标回收率法对该方法的准确性进行了验证。通过试验和分析,结果表明:异硫氰酸酯质量浓度在0.001~1 mg/mL,相关系数(r)大于0.999 9,线性较好,加标回收率在94.5%~102.0%之间。通过试验和分析,得出该方法具有高灵敏度、高准确性等优点,且操作简单,可以较快地得到测定结果,可有效地应用于饲料中异硫氰酸酯含量的测定与加标检测。The content of isothiocyanates in feed directly affects the growth and health of animals,so the rapid and accurate determination of isothiocyanates in feed has become an important task in agricultural production.This paper aims to establish a method for determining the content of isothiocyanate in feed using butyl isothiocyanate as the internal standard,so as to provide scientific basis and technical support for agricultural production and inspection and testing.In the experiment,cat food was selected as a sample,isothiocyanate in the feed was extracted,and it was analyzed using gas chromatography-mass spectrometry(GC-MS).At the same time,the accuracy of the method was verified by the spiked recovery method.Through experiments and analysis,the results showed that the isothiocyanate concentration was 0.001-1 mg/mL,the correlation coefficient(r)was greater than 0.9999,the linearity was good,and the recovery rate of spiked was 94.5%-102.0%.Through experiments and analysis,it was concluded that the method had the advantages of high sensitivity and high accuracy,and the operation was simple,and the measurement results can be obtained quickly,which could be effectively applied to the determination and spike detection of isothiocyanate content in feed.

关 键 词：异硫氰酸钾 气相色谱法 测定 饲料 

分 类 号：S816.17[农业科学—饲料科学]