黑蒜提取物通过调控PI3K/Akt/mTOR信号通路促进乳腺癌细胞凋亡  

Black Garlic Extract Promotes Apoptosis in Breast Cancer Cells Through PI3K/Akt/mTOR Pathway

作　　者：杨骐玮 黄聪玲 李芳 安冉 贾国辉[2] 刘瑞[2] YANG Qiwei;HUANG Congling;LI Fang;AN Ran;JIA Guohui;LIU Rui(Inner Mongolia Medical University Third Clinical Medical College,Baotou 014010,China;The Third Affiliated Hospital of Inner Mongolia Medical University,Baotou 014010,China;School of Life Sciences,Inner Mongolia University,Hohhot 010021,China)

机构地区：[1]内蒙古医科大学第三临床医学院,内蒙古包头014010 [2]内蒙古医科大学第三附属医院,内蒙古包头014010 [3]内蒙古大学生命科学学院,内蒙古呼和浩特010021

出　　处：《肿瘤学杂志》2024年第7期551-555,共5页Journal of Chinese Oncology

基　　金：中国金属学会冶金安全与健康分会健康卫生科研项目(jkws202042);内蒙古医科大学科技百万工程联合项目(YKD2020KJBW(LH)056)。

摘　　要：[目的]探究不同浓度的黑蒜提取物(black garlic extract,BGE)对人乳腺癌细胞系-7(human breast cancer cell line-7,MCF-7)生长的影响,并进一步探讨其作用的机制。[方法]通过应用噻唑蓝法(methyl thiazolyl tetrazolium,MTT)、5-溴脱氧尿嘧啶核苷(bromo-deoxyuridine,BrDU)法检测不同浓度(25、50、100 mg/mL)BGE对于乳腺癌细胞增殖的影响。应用流式细胞术(AnnexinⅤ-FITC/PI双染色法)检测不同浓度BGE对于乳腺癌细胞凋亡的影响。应用蛋白质印迹法(Western blot)检测磷脂酰肌醇3-激酶(phosphoinositide 3-kinase,PI3K)、p-PI3K蛋白激酶B(Akt)、p-Akt、哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)、p-mTOR蛋白的表达。应用SPSS 26软件进行单因素方差分析。[结果]MTT结果表明,经不同浓度BGE作用后,24 h BGE 25、50、100 mg/mL实验组细胞增殖率(64.14%±1.52%,56.06%±3.12%,53.39%±1.62%)低于对照组(71.79%±2.73%)(P<0.01),48 h实验组细胞增殖率(81.23%±3.45%,74.71%±3.58%,69.42%±2.06%)低于对照组(89.28%±1.44%)(P<0.01),72 h实验组细胞增殖率(87.38%±3.65%,82.96%±8.46%,76.69%±6.60%)低于对照组(95.12%±3.98%)(P<0.01)。流式细胞术检测发现BGE 25、50、100 mg/mL实验组细胞凋亡率(9.57%±0.97%,15.23%±1.21%,17.42%±1.89%)高于对照组(5.53%±0.52%)(P<0.01)。Western blot法检测发现实验组磷酸化的PI3K、Akt与m TOR蛋白的表达与对照组相比均受到抑制(P均<0.01)。[结论]BGE对乳腺癌细胞增殖有抑制作用,对其凋亡有促进作用,其作用机制可能与PI3K/Akt/mTOR通路相关。[Objective]To investigate the effects and mechanism of black garlic extract(BGE)on the growth of human breast cancer cells.[Methods]Human breast cancer MCF-7 cells were treated with BGE at concentration of 25,50 and 100 mg/mL,respectively.The proliferation and apoptosis of MCF-7 cells were detected by MTT,BrDU and flow cytometry(AnnexinⅤ-FITC/PI double staining)methods,respectively.The expression of phosphoinositide 3-kinase(PI3K),p-PI3K,protein kinase B(Akt),p-Akt,mammalian target of rapamycin(mTOR),and p-mTOR was detected by Western blot.One-way analysis of variance(ANOVA test)was applied with SPSS 26 software.[Results]MTT assay showed that the proliferation rate MCF-7 cells treated with BGE(25,50 and 100 mg/mL)was lower than that of the control group(for 24 h:64.14%±1.52%,56.06%±3.12%and 53.39%±1.62%vs 71.79%±2.73%,P<0.01;for 48 h:81.23%±3.45%,74.71%±3.58%and 69.42%±2.06%vs 89.28%±1.44%,P<0.01;for 72 h:87.38%±3.65%,82.96%±8.46%and 76.69%±6.60%vs 95.12%±3.98%,P<0.01).Flow cytometry showed that the apoptosis rate of the BGE groups was higher than that of the control group(for 48 h:9.57%±0.97%,15.23%±1.21%and 17.42%±1.89%vs 5.53%±0.52%,P<0.01).Western blot showed that expression of phosphorylated PI3K,Akt and mTOR proteins in the BGE groups were all inhibited compared with the control group(all P<0.01).[Conclusion]BGE may inhibit proliferation and promote apoptosis of breast cancer cell through the PI3K/Akt/mTOR pathway.

关 键 词：黑蒜提取物 乳腺癌细胞 增殖 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]