CILP2在子宫内膜癌组织中的表达及临床意义  

Expression and Clinical Value of CILP2 in Endometrial Cancer

作　　者：唐宁 杜红飞[1] 刘腾[1] 许颖[1] Tang Ning;Du Hongfei;Liu Teng;Xu Ying(Department of Clinical Laboratory,the First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,Sichuan,China)

机构地区：[1]成都医学院第一附属医院检验科,成都610500

出　　处：《肿瘤预防与治疗》2024年第9期731-747,共17页Journal of Cancer Control And Treatment

基　　金：国家自然科学基金(编号:82273574)。

摘　　要：目的:利用在线生物信息学数据库,通过组织水平实验验证CILP2基因在子宫内膜癌中的表达和临床意义,并分析CILP2基因表达与患者预后、免疫浸润之间的关系。方法:通过TIMER数据库获得CILP2在泛癌中的表达情况并用DriverDBv3数据库验证,利用R软件对从TCGA数据库下载子宫内膜癌项目中得到的552例子宫内膜癌和35例正常子宫内膜组织的数据进行差异表达分析,Human Protein Atlas数据库分析子宫内膜癌中CILP2的阳性表达情况,R软件对TCGA数据库下载的子宫内膜癌项目数据进行临床相关性分析,进一步利用免疫组织化学方法检测子宫内膜癌组织芯片中CILP2的表达情况;cBioPortal数据库得到CILP2基因的突变情况,GEPIA、UALCAN、Linkedomics三个数据库对CILP2高、低表达组进行生存期的分析,R软件获得单因素和多因素Cox分析及诊断性ROC分析结果;利用STRING数据库构建与CILP2相互作用的蛋白网络,Linkedomics数据库获取CILP2共表达基因,R软件对共表达基因进行GO和KEGG分析并可视化,TISIDB数据库分析CILP2与肿瘤浸润免疫细胞和免疫调节因子之间的关系。结果:子宫内膜癌患者组织中CILP2基因的mRNA表达水平和蛋白水平均较正常组织显著升高。免疫组织学的实验结果证实了CILP2在子宫内膜癌中异常高表达。cBioPortal突变分析发现CILP2在变异组中高表达,且变异组子宫内膜癌患者的生存时间明显低于未变异组;GEPIA、UALCAN、Linkedomics的生存分析也发现CILP2高表达组子宫内膜癌患者的生存期明显短于低表达组,同时,单因素和多因素Cox分析结果得到CILP2的高表达与患者不良预后有关。STRING在线分析结果表明,与CILP2相互作用的蛋白质参与了包括调控转录、脂质代谢、泛素蛋白修饰、免疫细胞的分化等生物学过程。GO和KEGG富集分析显示,在子宫内膜癌组织中CILP2共表达的基因可能参与泛素蛋白转移酶活性的阳�Objective:To validate the expression and clinical significance of CILP2 gene in endometrial carcinoma through tissue-level experiments by utilizing an online bioinformatics database,and investigate the relations of CILP2 gene expression to prognosis and immune infiltration.Methods:The expression of CILP2 in pancarcinoma was obtained from the TIMER database and validated using the DriverDBv3 database.Differential expression analysis of 552 cases of endometrial cancer and 35 cases of normal endometrial tissue data downloaded from the TCGA database was performed using R software.The Human Protein Atlas database was utilized to analyze the positive expression of CILP2 in endometrial cancer.Clinical correlation analysis on the endometrial cancer data downloaded from TCGA database was conducted using R software,and the expression of CILP2 in endometrial cancer tissue chips was further detected by immunohistochemical method.Mutation information of the CILP2 gene was obtained from cBioPortal database.GEPIA,UALCAN and Linkedomics databases were used to analyze survival time for high and low expression groups of CILP2.Univariate and multivariate Cox analyses as well as diagnostic ROC analysis results were obtained using R software.A protein network interacting with CILP2 was constructed using STRING database;co-expressed genes of CILP2 were retrieved from Linkedomics database;these co-expressed genes were analyzed and visualized by GO and KEGG path-ways using R software;finally,the relations of CILP2 to tumor-infiltrating immune cells and immune regulatory factors were analyzed using TISIDB.Results:The mRNA and protein levels of CILP2 gene in endometrial carcinoma tissue were significantly higher than those in normal tissue.Immunohistological results confirmed the abnormally high expression of CILP2 in endometrial carcinoma.cBioPortal mutation analysis showed that CILP2 was highly expressed in the altered group,and the survival time of endometrial cancer patients in the variant group was significantly lower than that in th

关 键 词：CILP2 子宫内膜癌 临床意义 免疫浸润 

分 类 号：Q74[生物学—分子生物学] R733.33[医药卫生—肿瘤]