TEDC2在HCC中的诊断预后价值及免疫浸润相关分析  

Diagnostic and prognostic value of TEDC2 in HCC and correlation analysis of immune infiltration

作　　者：周继[1] 朱思洵 刘辉 冯建涛 ZHOU Ji;ZHU Si-xun;LIU Hui;FEN Jian-tao(The Jiangyin Hospital of Chinese Medicine,Wuxi 214400,China)

机构地区：[1]江阴市中医院普外科,江阴214400

出　　处：《肝胆外科杂志》2024年第4期307-314,共8页Journal of Hepatobiliary Surgery

基　　金：无锡市卫建委重点项目(Z202202)。

摘　　要：目的分析微管蛋白ε和δ亚型复合物2(Tubulin epsilon anddelta complex2,TEDC2)对肝细胞癌(Hepatocellu-lar carcinoma,HCC)诊断和预后的价值及其与免疫细胞浸润的关系,探索TEDC2在HCC发生发展中的生物学功能和相关信号通路。方法从癌症基因组图谱和基因表达综合数据库中获取HCC相关RNAseq数据,通过R语言和统计学方法整合分析TEDC2的表达水平、诊断预后价值及与临床病理特征、免疫细胞浸润的关系。通过基因富集分析TEDC2在HCC中的生物学作用。结果与正常组织比较,TEDC2在HCC肿瘤组织中高表达(P<0.001),受试者工作特征曲线下面积为0.970,TEDC2高表达的HCC患者预后差(P=0.001),可作为判断HCC预后的独立因素。功能和通路富集分析结果显示,TEDC2与细胞周期、血管生成和脂肪酸代谢等密切相关。免疫细胞浸润分析发现TEDC2的高、低表达组间免疫细胞浸润水平比较,差异有统计学意义(P<0.001)。结论TEDC2可能是HCC潜在的致癌风险因子,与患者临床病理特征、预后及免疫细胞浸润有关,可能是HCC的诊断生物标志物和潜在免疫治疗靶点。Objective To analyze the value of Tubulin epsilon and delta complex 2(TEDC2)in the diagnosis and prognosis of Hepatocellular carcinoma(HCC)and its relationship with immune cell infiltration,and explore the biological functions and related signaling pathways of TEDC2 in the occurrence and development of HCC.Methods RNAseq data related to HCC were obtained from the Cancer Genome Atlas and Gene Expression Omnibus database.The expression level,diagnostic and prognostic value of TEDC2 and its relationship with clinicopathological features and immune cell infiltration were comprehensively analyzed by R language and statistical methods.The biological role of TEDC2 in HCC was analyzed by gene enrichment.Results Compared with normal tissues,TEDC2 was highly expressed in HCC tumor tissues(P<0.001),the area under the subject's working characteristic curve was 0.970,and HCC patients with high TEDC2 expression had poor prognosis(P=0.001),which could be used as an independent factor to judge the prognosis of HCC.The results of functional and pathway enrichment analysis showed that TEDC2 was closely related to cell cycle,angiogenesis and fatty acid metabolism.Immune cellinfiltration analysis showed that the difference of immune cell infiltration level between high and low TEDC2 expression groups was statistically significant(P<0.001).Conclusions TEDC2 may be a potential carcinogenic risk factor for HCC,which is related to clinicopathological features,prognosis and immune cell infiltration of patients,and may be a diagnostic biomarker and potential immunotherapeutic target for HCC.

关 键 词：肝细胞癌 微管蛋白ε和δ亚型复合物2 诊断预后 免疫细胞浸润 

分 类 号：R735.7[医药卫生—肿瘤]