Streamlined process for effective and strand-selective mitochondrial base editing using mitoBEs  

作　　者：Xiaoxue Zhang Zongyi Yi Wei Tang Wensheng Wei 

机构地区：[1]Changping Laboratory,Beijing 102206,China [2]Biomedical Pioneering Innovation Center,Peking-Tsinghua Center for Life Sciences,Peking University Genome Editing Research Center,State Key Laboratory of Protein and Plant Gene Research,School of Life Sciences,Peking University,Beijing 100871,China [3]Academy for Advanced Interdisciplinary Studies,Peking University,Beijing 100871,China

出　　处：《Biophysics Reports》2024年第4期191-200,共10页生物物理学报（英文版）

基　　金：supported by funds from the Beijing Municipal Science & Technology Commission (Z181100001318009);the National Science Foundation of China (NSFC31930016);the Beijing Advanced Innovation Center for Genomics at Peking University and the Peking-Tsinghua Center for Life Sciences (to W. Wei);the Fellowship of China Postdoctoral Science Foundation (to Z. Yi)

摘　　要：Mitochondrial base editing tools hold great promise for the investigation and treatment of mitochondrial diseases.Mitochondrial DNA base editors(mitoBEs)integrate a programmable transcriptionactivator-like effector(TALE)protein with single-stranded DNA deaminase(TadA8e-V106W,APOBEC1,etc.)and nickase(MutH,Nt.BspD6I(C),etc.)to achieve heightened precision and efficiency in mitochondrial base editing.This innovative mitochondrial base editing tool exhibits a number of advantages,including strand-selectivity for editing,high efficiency,and the capacity to perform diverse types of base editing on the mitochondrial genome by employing various deaminases.In this context,we provide a detailed experimental protocol for mitoBEs to assist others in achieving proficient mitochondrial base editing.

关 键 词：Mitochondrial DNA base editors(mitoBEs) Transcription-activator-like effector(TALE) DEAMINASE Nickase 

分 类 号：Q78[生物学—分子生物学]